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Treating ich

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Treating ich

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March 31, 2009

Researchers from the University of Georgia College of Veterinary Medicine have made an unexpected dual discovery that could open new avenues for treating Ichthyophthirius multifiliis, or "Ich", a single-celled protozoan parasite that commonly attacks freshwater fish.

With the aid of whole-genome sequencing, researchers found that Ich harbors two apparently symbiotic intracellular bacteria: Bacteroides, which are usually found free-living, and Rickettsia, which are obligate intracellular bacteria. The two bacteria represent new species.

It was the presence of Rickettsia DNA sequences found in the initial genome data that provided scientists with a clue that bacteria might live inside of Ich. Intracellular bacteria have been described in free-living ciliates such as Paramecium, but never in Ich, which is an obligate parasite.

"It was unexpected; it was stunning to find bacteria in Ich. And, it came about due to the genome sequencing," said Harry W. Dickerson, a co-author who has been studying Ich in the veterinary college for more than 20 years and a member of the UGA Center for Tropical and Emerging Global Diseases, which has a focus on parasitic diseases, primarily of humans. "Ich occurs world-wide and is one of the most common protozoon pathogens of freshwater fish. It is easily recognized by most aquarists, and fish farmers often are confronted with massive epizootic outbreaks to devastating economic effect."

Ich (which causes "white spot disease") is a ciliated protozoan parasite that bores into the skin and gills of fish where it feeds, destroying tissue and thereby blocking exchange of oxygen and carbon dioxide, usually leading to death of the host. Each parasite grows on the fish from roughly 40 microns, which cannot be seen by the naked eye, to approximately one millimeter in diameter, which can easily be seen as a white spot. The parasites leave the fish in about 5-6 days. Each cell then divides multiple times to produce up to 1000 more infective organisms. The entire life cycle takes about 6-7 days. With subsequent rounds of infection, the number of parasites continues to increase, and each wave of re-infection becomes more deadly than the last. By the second or third re-infection the fish population is usually overwhelmed and fish begin to die. Fish that survive mild infections can develop immunity.

There are currently no drugs or chemicals that kill Ich while it resides in the fish skin or gills; they can only kill Ich when the parasite is in the water, and therefore all current therapies require a cyclical re-treatment program.

The first major outbreak of Ich in North America was recorded at the Chicago World's Fair in 1893.Ich is a well-known problem for aqua-culturists, aquarium owners, pond owners, hobbyists and retailers of freshwater fish. People and birds can also carry the parasite, unknowingly, from pond to pond.

"Work to sequence the genome of this parasitic protozoan unexpectedly revealed that bacterial DNA sequences were also present," noted Craig Findly, one of the college's researchers on the project. "Following up this discovery led to our demonstration that two new species of intracellular bacteria use Ich as their host. We now need to determine if these intracellular bacteria play a role in infection."

Next, the researchers will try to determine what role the two organisms play in the physiology of Ich and whether Ich remain infective if the bacteria are removed. The scientists hope their finding takes them a step closer to developing better treatments for Ich.