DNA and protein electrophoresis results are documented with a digital camera (above) with built-in UV and white light trays. Permanent images may be printed on polymer film or captured to the attached PC for analysis. Image documentation software provides extensive analysis of results (below). In the anaerobic environment of the COY chamber one can work with air sensitive compounds and proteins, as in the growth of anaerobic crystals for x-ray crystallography. An incubator within the glove box is used for the growth of anaerobic bacteria. The BFF provides services and training in biomolecule expression and purification. We design and construct genes and produce proteins in high yields in an experimental approach tailored to your research or production objectives. Our flexible approach lets you select (1) our contract research in which we provide the services or (2) your personnel may work in the BFF under the supervision of our staff. Examples of the services we provide include the following: Molecular Biology Protein Expression Fermentation Downstream Processing Other Services Molecular Biology Preparation of DNA Genomic Plasmid Mini, midi, and mega scales Southern blots Restriction Endonuclease Analysis Preparation of RNA Prokaryotic Eukaryotic Northern blots Cloning Preparation of cDNA libraries Probe design and screening Gene isolation or gene synthesis Vector selection Codon optimization Double stranded sequence data * Mutagenesis PCR amplification Primer design Optimization of reaction conditions Cloning of PCR amplified products Proteomics 2-D gel electrophoresis Melanie 2-D analysis package Anaerobic COY chamber Oxygen monitor Incubator Ultracentrifugation Protein Expression Recombinant expression systems E.coli Mammalian Yeast Baculovirus Culture growth Cell disruption Centrifugation Protein purification Protocol development Chromatography systems FPLC ÅKTA explorer Preparative isoelectric focussing (ampholyte free) Scale-up protein production Protein characterization Mass spectrometry * UV-visible absorption spectroscopy Circular dichroism Thermostability studies Amino acid analysis ** N-terminal sequencing ** Inductively coupled plasma atomic emission spectrometry * Biological assays Western blots Fermentation Biomass production Experience with a wide range of organisms Bacteria, yeast, mycelial fungi, cell culture Batch, fed-batch, or continuous culture Wide range of volumes available 5L, 24L, 100L, 200L, 400L, 500L working volume 800L fermentor (delivery early 2002) Oxygen supplementation and control on all fermentors pH control on all fermentors All fermentors set up for anaerobic culture and unusual gas mixtures High temperature culture for thermophiles and hyperthermophiles HPLC, GC and other analytical tools available for process monitoring Cell or supernatant harvesting possible using continuous centrifugation or hollow fiber filtration Process Development Experience in batch, fed-batch and continuous culture process development Experience in statistical experimental design for process optimization Factorial design Response surface methods Suite of 5L fermentors available for rapid optimization of fermentation conditions Full on-line process control Full on-line data logging HPLC, GC and other analytical tools available for process monitoring Downstream process development possible Development of protein purification methods Product stability testing and formulation development Downstream Processing Fermentation harvesting Biomass recovery by centrifugation or hollow fiber micro- or ultra-filtration Process liquid recovery by centrfugation or hollow fiber micro- or ultra-filtration Cell homogenization Chemical/enzymic methods French Press Gaulin homogenizer Microfluidics microfluidizer (coming early 2002) Dynomill (available on a rental basis) Removal of product from cell extracts Concentration of process liquid Lyophilization Small- or process-scale ultra-filtration Spray drying * Protein purification Development of protein purification methods Scale-up of protein purification strategies for in-house production or technology transfer Wide range of protein purification hardware Isoelectric focussing FPLC ÅKTA explorer 100 for large-scale processes Gradifrac system Several analytical and prep-HPLC systems Batch purification possible in bioreactors Large number of chromatography columns available from analytical to process scale Sample preparation Formulation available to your specifications or developed in-house Shipping possible in a range of package sizes and types Overnight shipping Refridgerated shipping Freezing to -20C or -80C Lyophilization Spray-drying * Evaporation Other Services Seed banking Storage available at ­20C or ­80C Preparation of glycerol stock cultures Preparation of stock cultures to your specifications or developed in-house Lyophilization Storage of live strains, plasmids, process samples Analytical Wide range of analytical tools available Electrophoresis HPLC GC GC-MS * Amino acid analysis * N-terminal sequencing * Spectroscopy DNA sequencing * Nova Biomedical chemistry analyzer (available middle 2002) Analysis of a wide range of media components and metabolic products * UGA service facility ** Outside service facility Purpose of Lab . Description of Services . Lab Facilities . Lab Protocols . Fee Structure . Project Sign-up . Instrument Reservation . Bulletin Board . Advisory Board . Contact Info . H O M E