BFF Laboratory Instrumentation The BioXpress Lab is located in Labs 676, 677, and 678 of the Chemistry Building and in the Fermentation Facility on the University of Georgia campus. Download printable equipment/floor plans: Labs 676/678—Macintosh EPS format Labs 676/678—Windows EPS format Labs 676/678—PDF format (Acrobat file) Lab 677—Macintosh EPS format Lab 677—Windows EPS format Lab 677—PDF format (Acrobat file) Anaerobic Chamber 1 COY Laboratories Type A anaerobic chamber with Incubator Model 77 and O2 monitor . location Balances 1 Sartorius Analytical, Basic plus series, Sartorius No. BP210S . location . documentation 2 Sartorius Top loading, Basic plus series, Sartorius No. BP2100S . location . location . documentation 1 Ohaus Trip Balance (Harvard Trip Balance) . location . documentation Cell Disruptors 1 Fisher Scientific Sonic Dismembrator, Fisher Model 550 . location . documentation 1 Powergen 700 grinder . location 1 SLM-Aminco french press . location Centrifuges 1 Beckman GS-15R, refrigerated table top, 17.5 g maximum . location 1 Beckman Avanti J-25I high speed, high capacity . location . documentation 1 Beckman Optima TLX table top ultracentrifuge 3 Hereaus Biofuge Pico microcentrifuges . location . location . location Chromatography Columns (See Protein Purification below) 1 Superdex 75, HR 10/30, gel filtration 1 Superdex 200, HR 10/30, gel filtration . documentation 1 Superdex 75, high load 26/60, gel filtration 1 Superdex 200, high load 26/60, gel filtration 1 Mono Q HR5/5, analytical scale anion exchange 1 Mono Q HR10/10, preparative scale anion exchange 1 Mono S HR5/5, analytical scale cation exchange . documentation 1 Mono S HR10/10, preparative scale cation exchange . documentation 1 Resource Q, preparative anion exchange, 6 mL . documentation 1 Resource S, preparative cation exchange, 6 mL . documentation 1 Sephasil C18 peptide, reverse phase . documentation 1 Source RPC, reverse phase Cold Storage 2 -80°C Revco freezers . location . location 2 -20°C VWR freezers . location . location 1 Fisher Isotemp Chromatography refrigerator . location 3 VWR Refrigerator/freezer combination . location . location . location Computers and Printers 1 Power Mac 9600 Work Group Server . location 1 Power Mac 7100 1 Apple Color Laserwriter 12/660 postscript printer . location 1 Apple Laserwriter 16/660 postscript printer . location 2 Apple StyleWriter 6500 ink jet printers connected to FPLC system and Cary 50 spectrophotometer . location . location 2 Compaq DeskPro PCs connected to Cary 50 spectrophotometer and ImageMaster VDS . location . location 2 NEC PowerMate V2133 PCs connected to FPLC and ÅKTA systems . location . location 1 DELL 425 s/L PC connected to Cambridge Microplate reader . location Concentrators 1 Labconco CentriVap Evaporation system, a bench top concentrator Electrophoresis Systems 1 Amersham Pharmacia Biotech Phast system, proteins and nucleic acids . location 1 Hoefer Isoprime preparative isoelectric focussing (see Protein Purification) . location . documentation 1 Macrophor DNA/RNA/footprinting sequencing system . location 1 Multiphor II system for 2-D electrophoresis, proteomics . location . documentation Gel Rigs 4 Horizon 58, Mini submarine, (7.5 x 10) cm . location . location 2 HE 33 Mini submarine, (7 x 10) cm . location . location . documentation 2 GNA 100 submarine, (5 x 7.5) cm + (7.5 x 10) cm . location . location . documentation 4 GNA 200 submarine, full-size format, (20 x 20) cm . location . location . documentation 1 HE 100 Super Sub, large-format, field inversion . location . location . documentation Gel Dryer 1 SE1200 system . location . documentation Gel Slice Eluter 1 GE200 electrophoretic device . location . documentation Gel Stainer 1 Hoefer automated gel stainer, Coomassie or Silver stains, 19 programs . location . documentation Power Supplies 4 EPS 200, 200 V maximum . documentation 2 EPS 600, 600 V maximum . documentation 1 EPS 3500 XL high voltage, 3500 V maximum . location . documentation Transfer/Blotting 1 TE70 system . documentation 1 Schleicher & Schuell Turbo blotter Electroporator 1 BTX ECM 600 electroporator . location Hoods 1 Chemical Fume hood . location 1 Purifier-4 Class II, biosafety laminar flow . location Image Analysis 1 Image Master VDS system, Amersham Pharmacia Biotech . location . documentation 1 Melanie II 2-D electrophoresis, proteomics analysis software . location Incubator/Shakers 2 New Brunswick 4080, bench top, 4 L capacity each . location . location . documentation 2 New Brunswick 4335, floor model, refrigerated, programmable, 6 L capacity each . location . location . documentation Incubators/Ovens 3 Yamato digital ovens . location . location 1 Techne HB-2D hybridization oven . location 2 Microwave ovens . location . location Microplate Reader Model 7520 Cambridge Technology, Inc. . location PCR Thermal Cyclers 3 Thermolyne Amplitron II . location . documentation 1 Eppendorf Mastercycler gradient (on loan from the Prokaryotic Diversity RTG) . location pH Meter 1 Beckman Phi-310 pH/mV meter . location Plasmid Machine 1 MacConnell Mini-prep 24 automated plasmid mini-prep system Protein Purification Systems 1 Amersham Pharmacia Biotech FPLC 2826 . location 1 Amersham Pharmacia Biotech ÅKTA Explorer 10 mL, FPLC and HPLC . location . documentation 1 Hoefer PI 8 IsoPrime, preparative protein separation by isoelectric focussing, ampholyte free . location Pumps 2 P-1 Peristaltic single channel Rotator Boekel Oribtron Rotator Sequencing 1 Amersham Pharmacia Biotech Macrophor sequencing system Shaker 1 Orbital 361 . location . documentation Spectrophotometers 1 Cary 50 Bio UV-visible absorbance spectrophotometer with Peltier heating device . location . documentation . documentation 1 MBA 2000 UV-vis spectrophotometer with DNA/RNA/protein concentration calculators . location Ultrafiltration 2 Amicon 8400 ultrafiltration stirred cells, 400 mL capacity . location 2 Amicon 8050 ultrafiltration stirred cells, 50 mL capacity . location 2 Amicon 8010 ultrafiltration stirred cells, 10 mL capacity . location 1 Amicon MiniPlate YM3, tangential flow bioconcentrator, 3000 MWCO . location Ultrasonic Cleaner 1 Fisher FS30 . location UV Cross-linker 1 Fisher UVC500 . location . documentation Water Purification 2 Barnstead Nanopure Infinity UF systems . location . location . documentation Water Baths 1 Fisher Isotemp water bath . location . documentation 1 New Brunswick MDL C-76 water bath with shaker . location 1 Amersham Pharmacia MultiTemp III . location . documentation Purpose of Lab . Description of Services . Lab Facilities . Lab Protocols . Fee Structure . Project Sign-up . Instrument Reservation . Bulletin Board . Advisory Board . Contact Info . H O M E