Before you arrive at lab, please do the following: Read the Standard Operating Procedures for the EM Lab at www.uga.edu/caur/sop.htm Complete the online Right to Know safety module at UGA Env. Safety Division and bring the certificate to class. Download the Course Syllabus How to find the Center for Ultrastructural Research (EM Lab) and other information you need to work there. REFERENCES: To access the e-version of the book "Electron Microscopy" by Bozzola and Russell go to the UGA library site (http://www.libs.uga.edu/), and click on Galileo Database button. Search for author "bozzola" and it should provide several options, one of which is the Electron Microscopy textbook. Choose the e-version and it will pop up for you. You may need to "electronically" check out the book if you are spending too much time or flipping through several pages. The other text that will be helpful is available here as a PDF. It's called "Microscopy: Basics and Beyond" by Mortimer Abramowitz. This document will provide information on light and fluorescence microscopy. These PDFs on digitizing images and CCD cameras by Pawley are available to read for class Two PDFs are available from Oxford Instruments explaining EDS and WDS for x-ray analysis. As the class progresses, we will post helpful links to other sites below that will assist in your course work.

PowerPoint Presentations for class Safety in the Lab (Shields) Tomography(Keith) Brightfield and Phase Contrast Microscopy(Keith) Intro to EM (Wu) High Resolution TEM and STEM (Wu) Electron Microscopy Sample Preparation (Shields) Electron Diffraction (Keith) Extended Pressure Scanning Electron Microscopy (Shields) Fluorescence and Filters (Keith) Excel file of Fluorescent proteins list Lasers and Confocal Microscopy (Keith) Immuno Electron Microscopy and other labels (Shields) Multiphoton and Spectral Imaging (Keith) Dye Finder application notes from Leica --------End of section for First Exam ------------- Electron Microscope Optics (Shields) Beam-Specimen Interactions (Shields) SEM Design (Shields) SEM Sample Preparation (Shields) Structured illumination microscopy and reflection confocal (Keith) Cryopreparation and Cryo SEM (Shields) Image Processing (Keith) Cryo-TEM and Single Particle Reconstruction (Wu) WDS and EDS for EM (Shields)

LINKS:

Electron Microscopy
Electron Microscopy for Dummies - http://www.snaggledworks.com/em_for_dummies/
TEM Basics - http://www.matter.org.uk/tem/default.htm
Some basic EM techniques (neg staining, immuno labeling, etc...) - http://www.urmc.rochester.edu/research/emimg/techniqu.html
Negative Staining information - http://www.emlab.ubc.ca/Documents/Negative_Staining.htm
Online EM preparation protocols - http://www.protocol-online.org/prot/Image_Techniques/Microscopy/Electron_Microscopy/

Light and Laser Microscopy
The Teaching Modules at EMBL (EAMNET) - covers general history, FRET, FRAP and other analytical microscopy methods.
How to give a presentation and general talks on light and confocal microscopy.
Nikon MicroscopyU - http://www.microscopyu.com/
Laser Basics tutorial - http://members.aol.com/WSRNet/tut/ut1.htm
Glossary of Laser Basics - http://www.columbia.edu/cu/mechanical/mrl/ntm/Glossary.html
Fluorescent Dye spectra (interactive) - http://www.mcb.arizona.edu/IPC/spectra_page.htm

MicroCT (x-ray tomography)
Skyscan product site - http://www.skyscan.be/next/home.htm
Toshiba MicroCT with how it works - http://www.toshiba-itc.com/cat/en/prod02.html