Epifluorescence and Confocal Filter Sets available at the Center
Click here for chart of one-photon and two-photon fluorochrome database
Note: this is a database in progress and is subject to corrections, additions and modification.
There are three fluorescence microscopes available. Epifluorescence cube information provided by manufacturers (some Nikon filters manufactured by Chroma
1. Inverted Nikon TE300 with a Princeton CCD camera and conventional film camera attached
2. Nikon Optiphot attached to BioRad confocal system
3. Leica DMRX-E attached to Leica confocal system
Filter sets that can be used in either Nikon microscope are indicated with an asterisk.
| Filters for Nikon |
Excitation |
Dichroic/Barrier |
Emission |
| *UV (DAPI/Hoechst/AMCA) |
D 340-380 |
400 DCLP |
D 435-485 |
| *FITC/GFP |
D 465-495 |
505 DCLP |
D 515-555 |
| *FITC/GFP |
450-490 |
510 LP |
520-560 |
| *TRITC/rhodamine |
528-552 |
565 DCLP |
578-632 |
| *TRITC/rhodamine |
510-650 |
580 LP |
590 |
| Endow cube (GFP specific) |
HQ 450-490 |
Q 496 LP |
HQ 500-550 |
| Filters for Leica |
|
|
|
| DAPI/Hoecsht |
340-380 |
NA |
425 LP |
| FITC/GFP |
450-490 |
NA |
515 LP |
| FITC restricted |
460-500 |
NA |
512-542 |
| TRITC/rhodamine |
515-560 |
NA |
590 LP |
Filters for Confocal Laser Excitation
Note: Leica excitation/dichroic/emission detection is user defined
| BIORAD confocal cube |
Excit. |
|
| FITC/GFP |
488 |
|
| TRITC/Rhodamine |
568 |
|
| CY5/far red |
688 |
|
| LEICA laser |
Excit. |
Dichroics |
| Argon: |
458
478
488
514 |
RSP 500
458/514
RT 30/70
substrate |
| GreNe: |
543 |
488/543 |
| HeNe: |
633 |
488/543/633 |
| Two-photon laser tunable: 780-900 nm |
