LECTURE

August 17     Introduction and History of EM

22    Specimen Preparation:

24    Fixatives and Buffers

26

29

31

Sept. 3    LABOR DAY HOLIDAY

5    Dehydration and Embedding Resins

7    Sectioning and Staining

10

12    Support Films

14    Negative Staining

17    Critical Point and other Drying Techniques

19    Specimen Coating for SEM

21

24    FIRST HOURLY EXAM (100 pts.)

26    Electron Optics - Electron Sources

28    Electromagnetic and electrostatic lens theory

Oct. 1    Lens Aberrations

3    Vacuum Systems

5    Column Design: SEM vs. TEM

8    SEM Design

10    SEM Detectors

12    Beam Specimen Interactions - Secondary and backscatter electrons

15    Auger electrons and X-rays

17    X-rays: EDS vs. WDS

19    Electron Diffraction

22    Electron Spectroscopic Imaging and EELS

24    Specialized Preparation Techniques

25-26    FALL BREAK

29    Shadowcasts and Replicas

31    Cryopreservation

Nov. 2    Freeze Fracture

5    Immuno-EM

7    Cytochemistry

9    SECOND HOURLY EXAM (100 pts.)

12    Image Capture - Photography

14    Digital Imaging

16    Image Processing and Image Analysis

19

21-23    THANKSGIVING HOLIDAYS

26    Imaging and Output Devices

28

30    Confocal Microscopy

Dec.  3    Low Voltage vs. High Voltage EM

5    Scanning Probe Microscopy
 

10     (12-3 pm) FINAL EXAM (200 pts.)