Brief History of TEM
Introduction to TEM
Biological Specimen Preperation
- Goals
- Problems
Fixation
- Buffers
- Cacodylate
- Collidine
- HEPES
- Phosphate
- PIPES
- Tris
- Veronal Acetate
- Fixatives
- Formaldehyde
- Gluteraldehyde
- Acrolein
- Osmium Tetroxide
- Permanganate
- Diimdidoesters
- Methods of Fixation
- Dehydration
- Embedding Media
- Methacrylates
- Polyester Resins
- Epoxy Resins
- Alternative Embedding Media
- 10% Polystyrene
- LR White
- LR Gold
- Lowicryl
- Polyethylene Glycols
Support Films
Sectioning
- Problems
- Resin Polymerization
- Block Face
- Equipment
- Knives
- Sectioning Imperfections
- Knife Marks
- Compression
- Chatter
- Section Thickness
- Pick Up Sections
- Staining Sections
- Positive Staining
- Post-embedding Staining
Theory Of Electron Optics
- Rsolution
- Electromagnetic Lenses
- Electrostaic Lenes
TEM Lenses
- Condensor
- Objective
- Projector
Vacuum Systems
- Mechanical Pump
- Diffusion Pump
- Sputter-ion Pump
- Orbitron Pump
- Turbomolecular Pump
- Cryogenic Pump
Autoradiography
Electron Energy Loss Spectrscopy (EELS)
Electron Spectroscopic Imaging (ESI)
TEM Notes #2
Negative Staining
- Advantages
- Disadvantages
- General Procedure
Replicas and Shadowing
- Shadowing
- Heated Electrodes
- Electron Beam Evaporation
- Cathode Etching
- Shadow Cast
Cryopreservation
- Rapid Freezing
- Cryoprotectants
- Freeze Drying and Distillation
- Cryosectioning
- Freeze Fracture
Immunoelectron Microscopy
Enzyme Cytochemistry
Lectins
Stereology
X-Ray Microanalysis
- EDX
- WDS
Electron Diffraction
Intermediate and High Voltage EM
GoalsProblemsBuffers- Cacodylate
- Collidine
- HEPES
- Phosphate
- PIPES
- Tris
- Veronal Acetate
Fixatives
- Formaldehyde
- Gluteraldehyde
- Acrolein
- Osmium Tetroxide
- Permanganate
- Diimdidoesters
Methods of Fixation
Dehydration
Embedding Media
- Methacrylates
- Polyester Resins
- Epoxy Resins
- Alternative Embedding Media
- 10% Polystyrene
- LR White
- LR Gold
- Lowicryl
- Polyethylene Glycols
Problems
- Resin Polymerization
- Block Face
- Equipment
Knives
Sectioning Imperfections
- Knife Marks
- Compression
- Chatter
Section Thickness
Pick Up Sections
Staining Sections
- Positive Staining
- Post-embedding Staining
Rsolution
Electromagnetic Lenses
Electrostaic Lenes
Condensor
Objective
Projector
Mechanical Pump
Diffusion Pump
Sputter-ion Pump
Orbitron Pump
Turbomolecular Pump
Cryogenic Pump
Advantages
Disadvantages
General Procedure
Shadowing
- Heated Electrodes
- Electron Beam Evaporation
- Cathode Etching
Shadow Cast
Rapid Freezing
Cryoprotectants
Freeze Drying and Distillation
Cryosectioning
- Freeze Fracture
EDX
WDS