JUDITH H. WILLIS
Professor
Ph.D., 1961
Harvard University
RESEARCH
Two major problems in insect physiology remain fertile areas for analysis—the genetic basis for metamorphosis and the ways chitin and cuticular proteins interact to form the diverse types of cuticle found in a single insect in different regions and at different metamorphic stages. My research program combines these two areas. We have used cuticular proteins as molecular indictors of metamorphic stages to probe the action of the two hormones that control metamorphosis. We are studying the interaction of cuticular proteins with chitin using proteins expressed in vitro and by analyses of the structure and diversity of cuticular proteins.
Past research in my laboratory has focused on cuticular proteins in the giant silk moth, Hyalophora cecropia, and their regulation by juvenoids and ecdysteroids. My students and I have shown that some cuticular proteins are used in more than a single metamorphic stage while others are stage-specific. We have isolated and characterized the genes for two cuticular proteins that are expressed in more than one stage. Studies on their regulation indicate that both transcriptional and translational controls are involved. In the hope of identifying a more tractable experimental system, I am working with hormone-responsive cell lines derived from wing imaginal discs of the Indian meal moth and of Drosophila. If similarities are found between development in vivo and in vitro, we will have an excellent model system for learning how hormones control the complex macromolecular changes that accompany metamorphosis.
We have recently learned that a novel protein domain, found in many cuticular proteins in crustaceae, arachnids and insects, is necessary and sufficient for such proteins to bind to chitin. A new research thrust is to learn how many cuticular protein genes are found in a single species and to analyze in detail their patterns of expression. Anopheles gambiae has been selected for this study because its entire genome has been sequenced. As a result, numerous fundamental questions about metamorphosis and coordination of cuticular protein gene expression are now amenable to direct analysis.
| CONTACT INFORMATION |
| (706) 542-0802, jhwillis@cb.uga.edu |
OF NOTE
SUPPORT STAFF
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E-MAIL |
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| Hitoshi Tsujimoto | |
Grad Student | |
hittsuji@uga.edu |
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REPRESENTATIVE PUBLICATIONS
Cornman, R.S., T. Togawa, W. A. Dunn. N. He, A. C. Emmons, J. H. Willis. 2008. Annotation and analysis of a large cuticular protein family with the R&R Consensus in Anopheles gambiae. BMC Genomics 9:22.
Togawa, T., W. A. Dunn, A. C. Emmons, J. Nagao, and J. H. Willis. 2008. Developmental expression patterns of cuticular protein genes with the R&R Consensus from Anopheles gambiae. Insect Biochem. Molec. Biol. 38:508-519.
Cornman, R. and J. H. Willis. 2008. Extensive gene amplification and concerted evolution within the CPR family of cuticular proteins in mosquitoes. Insect Biochem. Molec. Biol. 38:661-676.
Togawa, T, Dunn, W.A., Emmons, A.C., Willis, J.H. 2007. CPF and CPFL, two related gene families encoding cuticular proteins of Anopheles gambiae and other insects. Insect Biochem. Molec. Biol. 37:675-688.
Karouzou, M.V., Spyropoulos, Y., Iconomidou, V.A Cornman, R. S., Hamodrakas, S. J., Willis, J. H. 2007.Drosophila cuticular proteins with the R&R Consensus: annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences. Insect Biochem. Molec. Biol. 37:754-760.
He, N., Botelho, J.M.C., McNall, R. J., Belozerov, V., Dunn, W.A., Mize, T., Orlando, R., Willis., J.H. 2007. Proteomic analysis of cast cuticles from Anopheles gambiae by Tandem Mass Spectrometry. Insect Biochem. Molec. Biol. 37:135-146.
The Honeybee Genome Sequencing Consortium 2006. Insights into social insects from the genome of the honeybee Apis mellifera Nature 443:931-949.
Iconomidou, V. A., J. H. Willis, S. J. Hamodrakas 2005. Unique features of the structural model of ‘hard’ cuticle proteins: implications for chitin-protein interactions and cross-linking in cuticle. Insect Biochem. Molec. Biol. 35:553-560.
Willis, J. H., V. A. Iconomidoiu, R. F. Smith, S. J. Hamodrakas 2005. Cuticular proteins. In: Comprehensive Molecular Insect Science ( L. I. Gilbert, K. Iatrou, S.S. Gill, eds.). Elsevier vol. 4:79-110.
Magkrioti, C. K., I. C. Spyropoulos, V. A. Iconomidou, J. H. Willis, S. J. Hamodrakas 2004. cuticleDB: a relational database of Arthropod cuticular proteins. BMC Bioinformatics 5:138
Willis, J. H. and J. S. Willis 2004. Metamorphosis In: Encyclopedia of Entomology (J.L. Capinera, ed.) Kluwer Academic Publishers
Gu, S. and J. H. Willis 2003. Distribution of cuticular protein mRNAs in silk moth integument and imaginal discs. Insect Biochem. Molec. Biol. 33:1177-1188.