The CTEGD Flow Cytometry Facility provides the research community with state-of-the-art flow cytometry expertise and instrumentation, including a Dako CyAn, a Becton Dickinson FACSCalibur, a Beckman Coulter Cytomics FC500, a Dako MoFlo and a Bio-Rad Bio-Plex.

  • Location: Paul D. Coverdell Buiding Room S166.
  • Hours: 8:00 AM to 5:00 PM.
  • Contact: Julie Nelson ph: 706-542-9474 email: jnelson@uga.edu.

Click here to use our WEB SIGNUP SHEET.

The charges for all our equipment (except the Bio-Plex*) are as follows:

  • Inside UGA - $50/hour
  • Non-UGA academic institutions - $75/hour
  • Corporate - $100/hour
  • There is an additional $75 setup fee for sorting with the MoFlo.
*The charges for the Bio-Plex are half those listed. The following is a list of the equipment available with a brief description of its capabilities and a list of fluorochromes suitable for use with each instrument. If you want to use a fluorochrome not listed, please contact Julie Nelson (see above for contact information).


Becton Dickinson Facscalibur 

The FACSCalibur ( Becton Dickinson ) flow cytometer can analyze cells or particles based on up to 6 different parameters (see list below) using two different laser excitation wavelengths simultaneously, 488 and 633. This is a very user-friendly instrument that trained individuals may use without assistance. Users must attend a training session before operating this instrument.

 488 nm excitation:

  • FL1 - 530/30 BP filter used for FITC, AlexaFluor 488, GFP, YFP, CFSE, Vybrant Green and PKH67
  • FL2 - 585/42 BP filter used for PE, PKH26, DsRed, Vybrant orange, PI
  • FL3 - 679 LP filter used for PerCP or PE-Cy5 (aka tricolor)
635 nm excitation:
  • FL4 - 661/16 BP filter used for APC or Alexa Fluor 633



Dako Cyan

The CyAn ( Dako ) has three laser excitation wavelengths, 405, 488 and 610, that are capable of analyzing 11 parameters simultaneously at speeds up to 20,000 events/second. This cytometer is useful for those searching for rare cells and those with applications requiring more than 4-5 colors to define a cell population. The CyAn is designed to be user friendly and most investigators are comfortable with it after one or more training sessions.


 488 nm excitation:

  • FL1 - 530/30 BP filter used for FITC, AlexaFluor 488, GFP, YFP, CFSE, Vybrant Green and PKH67
  • FL2 - 585/42 BP filter used for PE, PKH26, DsRed, Vybrant orange, PI
  • FL3 - 613/20 BP filter used for PE-TxRed (aka ECD), PI, AlexaFluor 594, PE-AF610
  • FL4 - 680/30 BP filter used for PE-Cy5 (aka Tricolor), PerCP, 7AAD
  • FL5 - 750 LP filter used for PE-Cy7
405 nm excitation:
  • FL6 - 450/50 BP filter used for AlexaFluor 405, Pacific Blue, Hoescht, DAPI
  • FL7 - 530/40 BP filter used for Pacific Orange
633 nm excitation:
  • FL8 - 665/20 filter used for APC or Alexa Fluor 633
  • FL9 - 750 LP filter used for APC-Cy7 or APC-AlexaFluor 750


Beckman Coulter Cytomics FC500

The Cytomics FC500 ( Beckman Coulter ) has two laser excitation wavelengths, 488 and 633, that can measure up to 5 colors depending on which laser is turned on (see below). This cytometer has the additional capability of "walk-away" operation. It has two carousels that may be loaded with up to 32 tubes each to be run automatically without user intervention. Users must attend a training session before operating this instrument.

488 nm excitation ONLY:
  • FL1 - 525 BP filter used for FITC, AlexaFluor 488, GFP, YFP, CFSE, Vybrant Green, PKH67
  • FL2 - 575 BP filter used forPE, PKH26, DsRed, Vybrant orange
  • FL3 - 620 SP filter used for PE-TxRed (aka ECD), PI, AlexaFluor 594, PE-AF610
  • FL4 - 675 BP filter used for PE-Cy5 (aka Tricolor), PerCP, 7AAD
  • FL5 - 755 BP filter used for PE-Cy7
488 nm AND 633 nm excitations:
  • FL1 - 525 BP filter used for FITC, AlexaFluor 488, GFP, YFP, CFSE, Vybrant Green, PKH67
  • FL2 - 575 BP filter used for PE, PKH26, DsRed, Vybrant orange
  • FL3 - 620 SP filter used for PE-TxRed (aka ECD), PI, AlexaFluor 594, PE-AF610
  • FL4 - 675 BP filter used for APC, AlexaFluor 633, AlexaFluor 647, Alexa Fluor 700
  • FL5 - 755 BP filter used for APC-Cy7, APC-AlexaFluor 750

Dako Moflo 

The MoFlo ( Dako ) cell sorter purifies or enriches populations by sorting cells or particles based on 11 different parameters using either or all of the following excitation wavelengths - UV, 488, or 633. The MoFlo can sort at speeds up to 60,000 events/second, and can sort single cells into any multi-well platform. Anything that can be visualized on a flow cytometer may be sorted. Researchers interested in using the MoFlo should contact Julie Nelson (see above for contact information) to schedule a consultation.

488 nm excitation:

  • FL1 - 530/40 BP filter used for FITC, AlexaFluor 488, GFP, YFP, CFSE, Vybrant Green and PKH67
  • FL2 - 570/40 BP filter used for PE, PKH26, DsRed, Vybrant orange, PI
  • FL3 - 630/30 BP filter used for PE-TxRed (aka ECD), PI, AlexaFluor 594, PE-AF610
  • FL4 - 670/30 BP filter used for PE-Cy5 (aka Tricolor), PerCP, 7AAD
  • FL5 - 740 LP filter used for PE-Cy7
UV excitation:
  • FL6 - 450/65 BP filter used for AlexaFluor 350, Hoescht, DAPI
  • FL7 - empty
633 nm excitation:

  • FL8 - 670/40 filter used for APC or Alexa Fluor 633
  • FL9 - 740 LP filter used for APC-Cy7 or APC-AlexaFluor 750

 Bio-Rad Bio-Plex

The Bio-Plex ( Bio-Rad ) protein array system is a flexible, easy-to-use assay based on the Luminex platform. Color-coded beads are conjugated with proteins or peptides to enable the identification of up to 100 different analytes per well of a 96 well plate. Various kits are available from different companies for use of this technology, and information on these kits is available upon request. The Bio-Plex system is designed to be user-friendly and researchers may use it on their own after a single training session.

CYTOMETRY REFERENCES

The Flow Cytometry Facility has an extensive library containing back and current issues of Cytometry and the following flow cytometry reference books are available for limited check out.

  • Current Protocols in Cytometry, published by John Wiley and Sons in affiliation with the International Society for Analytical Cytology
  • Practical Flow Cytometry (2nd, 3rd and 4th editions), Howard M. Shapiro
  • Flow Cytometry and Cell Sorting (2nd edition), edited by Myron R. Melamed, Tore Lindmo and Mortimer L. Mendelsohn
  • Flow Cytometry (Parts A and B) edited by Zbigniew Darzynkiewicz, J. Paul Robinson and Harry A. Crissman
  • Flow Cytometry, First Principles, Alice Givan
  • Flow Cytometry Protocols (2nd edition), edited by Teresa S. Hawley and Robert G. Hawley
  • Apoptosis Methods and Protocols, edited by Hugh J. M. Bradley

Data Analysis

We have software to fit most if not all of your analysis and publishing needs.

FlowJo (Treestar, Inc. Oregon) is a sophisticated software analysis package including post-acquisition compensation and easy to use graphics generation for publication. FlowJo may be used for analyzing all data generated by any of our flow cytometers.

ModFit (Verity Software House, Maine) is a great software package for analyzing DNA cell cycle, apoptosis and proliferation using data from any flow cytometer.

Multicycle (Phoenix Flow Systems, California ) is another software package for analyzing DNA cell cycle using data from any flow cytometer

We also have very good acquisition and analysis software on our flow cytometers:

  • Cellquest Pro on the FACSCalibur
  • Summit on the MoFlo and the CyAn
  • CXP on the Cytomics FC500.
  • Bio-Plex Manager on the Bio-Plex

Analysis support is free for interested researchers.


Flow Cytometry Web Resources

In addition to the Becton Dickinson , Beckman Coulter , Dako and Bio-Rad corporate sites, the following flow cytometry websites are a great resource for the latest research and protocols