Athens, Ga.
– The potential of gene therapy has long been hampered by the risks associated
with using viruses as vectors to deliver healthy genes, but a new University of Georgia study helps bring scientists
closer to a safe and efficient gene delivery method that doesn’t involve
viruses.
Assistant professor of chemistry Yan Geng and her colleagues
in the UGA Franklin College of Arts and Sciences have created a novel synthetic
gene vector that packages DNA into well-defined nanostructures that allow it to
efficiently deliver genes without triggering immune responses. The study, primarily
carried out by doctoral student Jennifer Haley, appears in the June issue of
the journal Molecular BioSystems and
also may have implications for cancer treatment and vaccine development.
“We’ve developed a very versatile approach to creating
synthetic gene delivery vectors,” said Geng, a Georgia Cancer Coalition
Distinguished Scholar and a researcher in the UGA Cancer Center. “Our approach is relatively simple
– using simple chemical reactions to create a new class of packaging molecules that wrap up genes on
their own – and has the potential to be very useful in real-world, clinical
applications.”
Gene therapy involves replacing abnormal, disease-causing
genes with normal genes. To do this, genetically modified viruses often are used.
The viruses do a remarkable job of inserting the new genes into hosts, Geng
said, but they’re inherently dangerous. So while the use of viruses as gene
delivery vectors has been efficient, it also has led to unexpected and tragic
complications, some of which were fatal. Synthetic vectors, which use synthetic
molecules to package genes, are generally safer than viral vectors, Geng said.
The downside is that they’re not nearly as efficient. For millions of years,
viruses have evolved into a small size, a rich variety of shapes – spherical,
disk-like, and sometimes long filaments, and sophisticated mechanisms to
facilitate the easy entrance of their DNA into cells.
“In nature, viruses are precisely self-assembled by their
coating proteins and genome,” Geng said. “We have to learn from nature and
engineer a safer yet efficient gene delivery system for medical use.”
Synthetically packaging long strands of DNA into compact, small
structures has long been a challenge, but Geng’s team has developed a unique combinative
self-assembly method that allows scientists to control precisely the size and
shape of the vector. The Geng team synthesized small peptides – which are short
chains of amino acids – that bind to genes and emulate natural proteins to
minimize potential immune reactions. The researchers then attach the small
peptides onto a biocompatible polymer scaffold to create a clustered effect.
The clustered peptides of the combined molecule will automatically assemble
with DNA, while the polymer wraps around the assembly, creating a protective
shell. The researchers have discovered that the assembly process is extremely
sensitive to the clustered arrangement of the gene-binding peptides. To change
the shape and size of the vectors, the researchers simply change the attachment
density of the peptides on the polymer scaffold, resulting in shapes that vary
from spherical to donut shaped to long filaments.
“These gene vectors also can be further conjugated with
targeting molecules, which will allow us to deliver the right genes to the
right spot in our body,” Geng added.
With the synthesis of the vector complete, the scientists
now plan to assess how effective it is in integrating genes into cancer cells.
Geng said her ultimate goal is to use tumor-suppressor genes to treat cancer.
Another possibility is to use the synthetic vectors to introduce genes that
boost the immune system.
“Our research is still at an early stage,” Geng said, “but
we’ve developed a very promising system.”
The research was funded by the UGA Research Foundation and
the Georgia Cancer Coalition.
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