Analytical
Research Services


Analytical Services, Research, Consultation
in Nutritional Biochemistry and Related Fields
 
Provided by the Poultry Science Research Laboratory
 
Poultry Science Research Laboratory (PRL)
Room 221, Livestock Poultry Building
Athens Campus, College Station
Government Identification #
DUNS: 080762250
CAGE CODE: 1L8Y7
 
Laboratory Director
Dr.Ronald D.  Etheridge
voice: 706-542-0999
fax: 706-542-1827
retherid@arches.uga.edu
Head Technician
Elaine Foster
voice: 706-542-0946
 
Areas of Specialization
List of Chemical Analyses
Pricing
 

Introduction
Over the years, a great deal of time has been devoted by research scientists toward developing a better understanding of the principles of nutrition and applying these principles toward producing a superior product. These accomplishments have been made with sound laboratory techniques and a well trained staff of scientists and technicians capable of providing the necessary expertise to accomplish the task.
 
The Poultry Research Laboratory ( PRL ), located in the Livestock Poultry Building, offers its analytical services and expertise to the research community of the College of Agriculture. Researchers and organizations outside the College of Agriculture and University community can be served on a specialized case basis.
 
The laboratory is designed to help support the many researchers who have a need for the same type of analytical services. The primary goal of the laboratory is to return quality analytical results as rapidly as possible, using the most up to date, and accurate methods and equipment available, for an economical fee.
 
There are five areas of specialization depending on the analysis:
 
 
The Poultry Science Laboratory ( PRL ) is an interdepartmental service, research, and teaching facility. The laboratory performs over 40 different analytical procedures, from the standard proximate analyses on feed samples to sophisticated determinations on fatty acids using gas chromatographic techniques. The PRL also has the capability of setting up additional analyses that a researcher may need.

Whenever possible, the analytical methods used are official methods specified by the appropriate societies such as the AOAC. When official methods are not available, the procedures are in-house developed and validated. Quality control is part of our daily operations, utilizing both external and internal controls to assure the highest quality of performance of personnel, analytical systems, and data retrieval.

These analytical services are also available to enable a researcher to initiate a research project as soon as he/she comes on board. To begin the analytical phase of a research project in the PRL, one does not have to purchase all of the equipment, set it up, research a technique, and hire an train a technician. All that is required is that you have a representative sample of the material to be analyzed.

Students visit the laboratories in undergraduate courses and participate in analytical determinations in graduate course work.

The laboratory can furnish you information on your sample within a minimum of time, serve as a source of analytical information and help with the training of your graduate student. The staff is available to answer question concerning submitting samples for analysis, sample preparation, preservation and storage.

If you would like to visit our facility or find out more about our operation, please give me a call, Ron Etheridge (706) 542-0999.

 
SERVICES OFFERED
The Proximate Analyses:
A system for approximating the value of a feed for feeding purposes, without actually using the feed in feeding trials, was developed at the Weende Experiment Station in Germany over 100 years ago. It is based on the separation of feed components into fractions according to their feeding value. The various fractions in a proximate analysis are moisture, ash, protein, fat, and crude fiber.
 
Moisture is determined by the loss in weight of samples dried in a 105 degree centigrade oven.
Ash is the mineral component of a sample determined by burning the sample in a muffle furnace at 600 degrees centigrade for 2 hours.
 
Crude Protein is determined by the Kjeldahl Or Dumas combustion technique which determines the amino nitrogen of a sample.
 
Crude Fat is that portion of a sample that is soluble in diethyl ether. This represents total fat in most samples.
 
Crude Fiber is that portion of the moisture and fat free sample that remains after digestion with weak acid and base. It is of low digestibility and composed of cellulose, hemi-cellulose and some lignins.
 

Gas Chromatography

The gas chromatograph separates compounds on the basis of their solubilities in a liquid phase attached to a solid support. The sample is injected into the gas chromatograph where it is volatilized and transported by a carrier gas through a separation column. It is then burned is a flame ionization detector, producing an electronic signal which is directly proportional to the concentration of the materials present. The PRL uses the gas chromatograph to determine the fatty acid composition of fats from different sources such as tallow, milk, butter, and feed materials. In addition the volatile fatty acid profiles of rumen fluid, fecal material and blood can also be analyzed. Call us if you need some GC work, we will use your column or purchase one that will separate the substance of interest.

 

Mineral Analyses

Samples for mineral analysis are subjected to various treatments for solubilization, such as dry ashing, reagent extractions, and wet ashing using a variety of acid cocktails. The samples minus their organic material are analyzed using techniques such as atomic absorption, atomic emission, and colorimetric procedures. In atomic absorption the digested liquid sample is aspirated into a flame to produce free atoms of the element and the light absorbed is measured with a photometer. The amount of light absorbed at the specific wavelength of the metal is directly proportional to the concentration of the element. The concentration of 72 elements can be determined in trace amounts. In our laboratory some of the most requested analysis are for calcium, phosphorus, zinc, copper, magnesium, manganese and cadmium. Other elements of interest such as sodium, potassium and lithium can be determined by using the atomic absorption unit as an emission spectrophotometer. The technicon auto analyzer utilizes colorimetric procedures for mineral determination.

 

Special Chemical Analyses

The PRL has the capability of setting up special analytical procedures for researchers. Energy is a routine special chemical analysis using the bomb calorimeter to measure the calorie content of samples by measuring the heat produced by the sample upon ignition inside a steel bomb. Other chemical analysis such as total lipid, glycogen, chromic oxide, free fatty acids and lactic acid are available. Special chemical analyses for not so routine procedures have been set up for phytin phosphorus, acid insoluble ash and typsin inhibitor. For any special analytical procedure or problem please give me a call. Ron Etheridge 706-542-0999.

 

Clinical Chemistry

Serum or plasma samples are analyzed using the technicon auto analyzer continuos flow systems. These systems automate traditional wet chemical methods releasing the technician to perform other duties. Calcium and phosphorus in bones, and blood components such as glucose are analyzed using technicon procedures.


List of Chemical Analyses
 
Primary Nutrient Analysis: 
Moisture ( 105 degree C ,convection oven ) 
Moisture ( 70 degree C ,vacuum oven ) 
Moisture ( 60 degree C, forced air ) 
Protein- Nitrogen ( Dumas combustion ) 
Ash 
Ether Extract 
Crude Fiber 
Acid Insoluble Ash 
  
Mineral Analyses: Atomic absorption 
Copper 
Zinc 
Iron 
Magnesium 
Molybdenum 
Cobalt 
Lithium 
  
Mineral Analyses: Atomic Emission 
Sodium 
Potassium 
Mineral Analyses: Technicon Auto Analyzer 
Calcium 
Phosphorus 
  
Other Minerals: 
Chloride ( Silver Titration ) 
Chromic oxide ( Fusion NaOH ) 
  
Special Chemical Assays 
Total lipid ( fat ) 
Energy ( bomb calorimetry ) 
Fatty acid profile ( GLC ) 
Volatile Fatty Acids ( GLC ) 
Glycogen 
Phytin Phosphorus 
ph ( hydrogen ion concentration ) 
Trypsin Inhibitor 
Urease 

 

Pricing:

The PRL is a self supporting facility. The cost for analytical services depends on the cost of chemical reagents, type of analysis, type of sample, number of samples , and sample preparation required. The samples can be analyzed in single or duplicate replications, including a free repeat if results do not check within quality control guidelines. Volume discounts are available and questions concerning quantity of sample required for the analysis and sample preparation will be discussed during the initial conversation.

For price information contact Ron Etheridge 706-542-0999.
 retherid@arches.uga.edu

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