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P.F.G.E.
Croc DNA
Information
Repository
Conservation
Genetics of
Red Pandas
Protocols
written,
modified, or used in the
SREL DNA Lab
Our Protocols
Microsatellites
- Travis Glenn's LATEST & BEST PROTOCOL - Glenn&Schable_update2.pdf
- That update derives from "Glenn, T.C. and N.A. Schable. 2005. Isolating microsatellite DNA loci. Methods in Enzymology 395:202-222". - Glenn&Schable_MIE_05.pdf.
- Please contact Travis Glenn for any additional updates.
For ARCHIVAL/HISTORICAL PURPOSES ONLY [Do NOT Use Anymore!] see:
Glenn and Schable update 1 (strip tube sequencing) - Glenn&Schable_update1.pdf.
Travis Glenn et al.'s 3rd generation microsatellite isolation protocol - Msat_Easy_Isolation_2000.rtf
Guidelines for submitting microsatellite loci to Genbank - Genbank_STS_submissions.rtf
Travis Glenn's last attempted update for the whole darn thing - MsatMan2000.rtf
Pam Svete's 1999 "tweener" microsatellite isolation protocol - PSvete_MsatsManual.htm
Travis Glenn's "original" microsatellite protocol - MsatManV6.rtf
General Protocols
-- Cycle
sequencing set-up & EtOH precip optimized for strip tubes -
SREL_Sequencing_Protocol.rtf
-- PEG Cleaning of PCR products prior to sequencing - PEG_Preip'00.rtf
-- DNA Purification using MUD - MUD_DNA'00.rtf
Old
Protocols from the Smithsonian
-- Getting DNA out of old dead stuff - OldDNA99.rtf
-- Silica-based (MUD) extraction of DNA - TSilicaDNA.rtf
-- Single Strand Conformation Polymorphisms - SSCP'96V2.rtf
--
Plasmid Purification - Pounds-O-Plasmids'93.rtf
Modified Protocols
--
Chelexing - Quick & dirty DNA from fish fins or any other
tiny
amount of tissue - KariChelex_mod'00.rtf
Others'
Protocols
Microsatellites
Hamilton et al.'s Protocol
General Protocols
