The microsat-L list serve was initiated in the early 1990's by Tom Chapman at Simon Fraser University. Tom maintained the list until September 2000. Stevan Springer managed the list from September 2000 until June 2003. Chris Hill at Coastal Carolina University currently maintains the list of more than 500 active members. To reduce spam Tom actively managed. However, there are now much better automated spam filters, so Chris is returning to an automated format.
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We are putting together an archive of old questions & answers. Hopefully, we'll have that in a FAQ format in the reasonably near future. We are also putting together some general information that would be of interest to many list subscribers. This will be reorganized over time, but for the time being, this will get things started. If you know of any additional web sites that fit into these categories - send an e-mail to Travis Glenn (Travis.Glenn@sc.edu):
Microsatellite Development Protocols:
Many protocols to develop and score microsatellite DNA loci have been developed over the years. These protocols are available on the web. They are listed alphabetically by the first author's last name for your information only - we do not recommend any one approach over any other.
Cordeiro et al. 1999 - Optimisation of a Microsatellite Enrichment Technique in Saccharum spp.
Estoup & Turgeon's Microsatellite Protocols (http://www.inapg.inra.fr/dsa/microsat/microsat.htm)
Fisher & Bachmann's Protocol (http://barley.ipk-gatersleben.de/~dirkf/home.html)
Send Travis Glenn an e-mail & he'll send you a copy of the protocol currently used in his lab, which is not yet posted to their protocols web page. Older versions of his lab's protocols are posted on their protocols web page.
Travis Glenn's Microsatellite Manual developed at the Smithsonian (MsatManV6.rtf).
Travis Glenn's Microsatellite Manual2 developed at SREL by Ryan Thum and Pam Svete Updated Techniques, Summer 1999 .
TL Maguire's - Producing and Exploiting Enriched Microsatellite Libraries
Rob Toonen's Protocol (http://biogeek.ucdavis.edu/Msats/index.html)
Microsatellite DNA Development Labs
Several labs develop microsatellites on a commercial or semi-commercial basis. These are listed alphabetically for your information only - we do not recommend any one approach or lab over any other.
Academic:
Centre for Plant Conservation Genetics, Southern Cross University, Lismore NSW, Australia - http://www.scu.edu.au/research/cpcg/sxn2/cpcg_about.php
Genetic Analysis laboratory - part of Biotechnologies for the Ecological, Evolutionary and Conservation Sciences, University of Florida - http://www.biotech.ufl.edu/GeneticAnalysis/services.html
Savannah River Ecology Lab (SREL) University of Georgia - http://www.uga.edu/srel/Msat_Devmt/Microsatellites--home.htm
Sheffield Molecular Genetics Facility, University of Sheffield, UK - http://www.shef.ac.uk/misc/groups/molecol/smgf.html
Commercial:
Amplicon Express, Pullman Washington, USA - http://www.genomex.com/AEX_zone/index.html
Aquatic Stock Improvement Company (ASICo), Hawthorne California, USA - http://www.asicoaquaticmarkers.com/AHome2.htm
Bioprofiles Ltd., Newcastle, UK - http://www.bioprofiles.co.uk/
Ecogenics, Zurich, Switzerland - http://www.ecogenics.ch/
Genetic Identification Services (GIS), Chatsworth, California, USA - http://www.genetic-id-services.com/library.htm
Analysis of Chromatograms to obtain Microsatellite DNA Genotypes
A few companies and labs have developed software to analyze output from automated DNA sequencers to obtain genotype data from microsatellite DNA loci. These are listed for your information only - we do not recommend any one approach over any other.
Allelogram - Mac Based program for binning and normalizing microsatellites by Carl Manaster
Amersham Biosciences - for their MegaBase capillary instruments
Applied Biosystems - GeneScan, Genotyper, & now GeneMapper - for their gel and capillary instruments.
Beckman - for their CEQ 8-capillary instruments
Licor - SAGAGT for their gel-based instruments
MJ Research - Cartographer - for their BaseStation gel-based instrument
Spectrumedix - for their Aurora capillary machines
STRand - UC Davis Open source software compiled for Windows for genotyping from all ABI & BaseStation instruments
Analysis of Microsatellite DNA Genotypes
Several labs develop software to analyze genotype data from microsatellite DNA loci. These sites include links to many different packages that may be of interest.
Top sites found through google - http://directory.google.com/Top/Science/Biology/Genetics/Software/
Australia National University's Population Genetic Analysis Resources (including Genetic Analysis in Excel) - http://online.anu.edu.au/BoZo/GenAlEx/PGA_list.html
David McDonald's web site at the University of Wyoming - http://uwadmnweb.uwyo.edu/zoology/mcdonald/molmark/Data/WebSoft.html
For more information, contact: Chris Hill (chill@coastal.edu) or Travis Glenn (Travis.Glenn@sc.edu)
Last updated: June 29, 2005