Development and Use of Microsatellite DNA Loci for Genetic Ecotoxicological Studies of the Fathead Minnow (Pimephales promelas)

SREL Reprint #2541

Development and Use of Microsatellite DNA Loci for Genetic Ecotoxicological Studies of the Fathead Minnow (Pimephales promelas)

MARIANNA C. L. BROWN^1,2, SHELDON GUTTMAN¹ AND TRAVIS C. GLENN^2,3

¹Department of Zoology, Miami University, Oxford, OH 45056, USA
²Savannah River Ecology Laboratory, Drawer E, Aiken SC 29802, USA
³Department of Biological Sciences, University of South Carolina, Columbia SC 29208, USA

Accepted 17 December 2000

Abstract. The development of new DNA technologies has increased the opportunities to study the effect of genetic variation as a factor in selection, resistance, and fitness of individuals and populations. Genetic composition affects the evolutionary potential of an organism; however, whether genetic variation has a role in adaptation and survival due to chemical stress is still under debate. The strategy of measuring such variation should be based on the desired number of loci needed and degree of polymorphism at each locus. Microsatellite DNA loci (also known as Simple Tandem Repeats, STRS) have among the highest levels of variation within most eukaryotic genomes. Methods to determine the high levels of variation in individuals and populations have become more refined, making DNA-based studies in ecotoxicology more feasible. Herein, we describe the general characteristics of microsatellite DNA loci, their isolation, and their applications to toxicological studies using the fathead minnow (Pimephales promelas) as a focal species.

Keywords: allele; genetic markers; microsatellites; resistance; STR

SREL Reprint #2541

Brown, M. C. L., S. Guttman, and T. C. Glenn. 2001. Development and use of microsatellite DNA loci for genetic ecotoxicological studies of the fathead minnow (Pimephales promelas). Ecotoxicology 10:233-238.

To request a reprint


SREL Reprint #2541 *Development and Use of Microsatellite DNA Loci for Genetic Ecotoxicological Studies of the Fathead Minnow (Pimephales promelas)* MARIANNA C. L. BROWN^1,2, SHELDON GUTTMAN¹ AND TRAVIS C. GLENN^2,3 ¹Department of Zoology, Miami University, Oxford, OH 45056, USA ²Savannah River Ecology Laboratory, Drawer E, Aiken SC 29802, USA ³Department of Biological Sciences, University of South Carolina, Columbia SC 29208, USA Accepted 17 December 2000 Abstract. The development of new DNA technologies has increased the opportunities to study the effect of genetic variation as a factor in selection, resistance, and fitness of individuals and populations. Genetic composition affects the evolutionary potential of an organism; however, whether genetic variation has a role in adaptation and survival due to chemical stress is still under debate. The strategy of measuring such variation should be based on the desired number of loci needed and degree of polymorphism at each locus. Microsatellite DNA loci (also known as Simple Tandem Repeats, STRS) have among the highest levels of variation within most eukaryotic genomes. Methods to determine the high levels of variation in individuals and populations have become more refined, making DNA-based studies in ecotoxicology more feasible. Herein, we describe the general characteristics of microsatellite DNA loci, their isolation, and their applications to toxicological studies using the fathead minnow (Pimephales promelas) as a focal species. Keywords: allele; genetic markers; microsatellites; resistance; STR SREL Reprint #2541 Brown, M. C. L., S. Guttman, and T. C. Glenn. 2001. Development and use of microsatellite DNA loci for genetic ecotoxicological studies of the fathead minnow (Pimephales promelas). Ecotoxicology 10:233-238. To request a reprint