SREL
Reprint #2612
Cross-species
amplification among peromyscines of new microsatellite DNA
loci from the oldfield mouse (Peromyscus polionotus subgriseus)
KELLY
L. PRINCE,1,2 TRAVIS C. GLENN,2,3
and MICHAEL J. DEWEY1,2
1Peromyscus Genetic Stock Center, University
of South Carolina, Columbia, SC 29208, USA
2Department of Biological Sciences, University
of South Carolina, Columbia, SC 29208, USA
3Savannah River Ecology Laboratory, P.O.Drawer
E, Aiken, SC 29802, USA
Abstract
We describe polymerase
chain reaction (PCR) primers and conditions to amplify 11
microsatellite DNA loci isolated from the oldfield mouse
(Peromyscus polionotus subgriseus). These were tested
for amplification using nine species and subspecies maintained
at the Peromyscus Genetic Stock Center, with an average
success rate of 65% and two loci amplifying in all species.
Polymorphism was tested within the P. polionotus subgriseus
and the recently obtained P. maniculatus sonorensis colonies.
P. p. subgriseus had modest numbers of alleles per
locus (1-4), whereas P. m. sonorensis had many alleles
per locus (5-10) and high expected heterozygosities (0.625-0.878).
Keywords:
dinucleotide repeats, PCR, Peromyscus, primer, Rodentia,
SSR
SREL
Reprint #2612
