Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin)
J.
SUSANNE HAUSWALDT1,2 andTRAVIS C. GLENN1,2
1Department of Biological Sciences, University
of South Carolina, Columbia, SC 29208, USA
2Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC
29802, USA
Abstract
We describe polymerase chain recation (PCR) primers and conditions to
amplify one dinu- cleotide and five tetranucleotide microsatellite DNA
loci isolated from the Diamondback terrapin (Malaclemys terrapin),
The PCR primers were tested on 21 terrapins from Cape Romain, SC, USA.
The microsatellite primers developed yielded a high number of alleles
(8-14) and high observed heterozygosities (0.57-1.0).
Keywords: dinucleotide repeats, Malaclemys terrapin,
microsatellites, primer, SSR, tetranucleotide repeats
SREL Reprint #2690
Hauswaldt, J. S. and T. C. Glenn. 2003. Microsatellite DNA loci from the diamondback terrapin (Malaclemys terrapin). Molecular Ecology Notes 3:174-176.
