Development of microsatellite DNA loci from the wood stork (Aves, Ciconiidae, Mycteria americana)
ALESSANDRA
M. TOMASULO-SECCOMANDI1, NANCY A. SCHABLE2,
A. LAWRENCE BRYAN, JR2, I. LEHR BRISBIN, JR2, SILVIA
N. DEL LAMA1 and TRAVIS C. GLENN2
1 Universidade Federal de Sao Carlos, Depto. Genetica e Evolucao-Sao
Carlos, SP, CEP 13565-905, Brazil
2Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC
29802, USA
Abstract
We isolated 11 polymorphic microsatellite loci for wood stork (Mycteria
americana). Polymerase chain reaction (PCR) primers and conditions
are described for the amplification of five dinucleotide, one trinucleotide
and five tetranucleotide microsatellite loci. The PCR primers were tested
on two wood stork populations, Fazenda Ipiranga, Mato Grosso, Brazil (n
= 11) and Tamiami West, Everglades, Florida, USA (n = 20). The
primers yielded two to four alleles per locus, an observed heterozygosity
of 0.0-0.727 and a polymorphic information content of 0.048-0.604. The
low level of polymorphism for these markers is consistent with previous
studies of this species.
Keywords: dinucleotide repeats, microsatellite, Mycteria
americana, primer, SSR, tetranucleotide repeats, trinucleotide repeats,
wood stork
SREL Reprint #2710
Tomasulo-Seccomandi, A. M., N. A. Schable, A. L. Bryan, Jr., I. L. Brisbin, Jr., S. N. Del Lama and T. C. Glenn. 2003. Development of microsatellite DNA loci from the wood stork (Aves, Ciconiidae, Mycteria americana). Molecular Ecology Notes 3:563-566.
