Seven polymorphic microsatellite DNA loci from
the red-spotted newt (Notophthalmus viridescens)
DEAN
A. CROSHAW1,2 and TRAVIS C. GLENN1,3
1 Savannah River Ecology Laboratory, University of Georgia,
Drawer E, Aiken, SC 29802, USA
2 Department of Biological Sciences, University of New Orleans,
New Orleans, LA 70148, USA
3 Department of Biological Sciences, University of South Carolina, Columbia,
SC29208, USA
Abstract
We describe polymerase chain reaction (PCR) primers and amplification
conditions for seven microsatellite DNA loci isolated from the red-spotted
newt (Notophthalmus viridescens). Primers were tested on 16 individuals
from two populations on the Savannah River Site in Aiken County, South
Carolina. We detected six to 10 alleles per locus and an overall observed
heterozygosity range of 0.31-0.81. Despite low heterozygosity at two of
the seven loci, the high polymorphic information contents (from 0.54 to
0.85) of these markers render them useful for future studies of the behavioural
and population ecology of this common salamander.
Keywords: microsatellites, newts, Notophthalmus viridescens,
PCR primers, SSR, STR
SREL Reprint #2711
Croshaw, D. A. and T. C. Glenn. 2003. Seven polymorphic microsatellite DNA loci from the red-spotted newt (Notophthalmus viridescens). Molecular Ecology Notes 3:514-516.
