Polymorphic tetranucleotide microsatellite DNA
loci from the southern dusky salamander (Desmognathus auriculatus)
DEAN A. CROSHAW1,2 and TRAVIS C. GLENN1,3
1 Savannah River Ecology Laboratory, University of Georgia,
Drawer E, Aiken, SC 29802, USA
2 Department of Biological Sciences, University of New Orleans,
New Orleans, LA 70148, USA
3 Department of Biological Sciences, University of South Carolina,
Columbia, SC29208, USA
Abstract
We describe polymerase chain reaction (PCR) primers and amplification conditions for seven tetranucleotide microsatellite DNA loci isolated from the southern dusky salamander (Desmognathus auriculatus). Primers were tested on 16 individuals from one population in Aiken County, South Carolina. We detected an average of 6.57 alleles per locus, an observed heterozygosity range of 0.44-0.94, and high polymorphic information contents (mean of 0.68).
Keywords:
Desmognathus auriculatus, dusky salamanders, microsatellites,
polymerase chain reaction primers, short tandem repeat, simple sequence
repeat
SREL Reprint #2712
Croshaw, D. A. and T. C. Glenn. 2003. Polymorphic tetranucleotide microsatellite DNA loci from the southern dusky salamander (Desmognathus auriculatus). Molecular Ecology Notes 3:623-625.
