Microsatellite loci isolated from narrow-leaved cattail Typha augustifolia
OLGA V. TSYUSKO-OMEiTCHENKO,
NANCY A. SCHABLE,* MICHAEL H. SMITH and TRAVIS C. GLENN
The University of Georgia, Institute of Ecology, Savannah
River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA
Abstract
We present 11 dinucleotide micro satellite DNA loci isolated from the narrow-leaved cattail (Typha angustifolia) and describe conditions for their amplification. The PCR primers were tested on at least 20 individuals of Typha angustifolia and T. latifolia from two Ukrainian populations per species. The primers amplify loci with relatively high numbers of alleles (averaging 7.22 and 4.95 alleles per locus in T. angustifolia and T. latifolia, respectively); and polymorphic information content (averaging 0.61 and 0.46 in T. angustifolia and T. latifolia, respectively).
Keywords: dinucleotide repeats, microsatellite, PCR,
primer, radiation, Typha angustifolia, Typha latifolia, Ukraine
Received 8 April 2003; revision received 18 June 2003; accepted 13 July
2003
SREL Reprint #2725
Tsyusko-Omeltchenko, O. V., N. A. Schable, M. H. Smith and T. C. Glenn. 2003. Microsatellite loci isolated from narrow-leaved cattail Typha angustifolia. Molecular Ecology Notes 3:535-538.
