Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus)
NANCY A. SCHABLE,1,3 BRANT c. FAIRCLOTH,2 WILLIAM
E. PALMER,4 JOHN P. CARROLL,2 L. WES BURGER,5
LEONARD A. BRENNAN,6 CRIS HAGEN1 andTRAVIS C. GLENN1,3
1Savannah River Ecology Laboratory, University
of Georgia, P.o. Drawer E, Aiken, SC 29802, USA,
2D. B. Warnell School of Forest Resources, University of Georgia,
Athens, GA 30602, USA,
3Department of Biological Sciences, University of South Carolina,
Columbia, SC 29208, USA,
4Tall Timbers Research Station, 13093 Henry Beadel Drive, Tallahassee,
FL 32312, USA,
5Department of Wildlife and Fisheries, Box 9690, Mississippi
State University, Mississippi State, MS 39762-9690, USA,
6Richard M. Kleberg Jr Center for Quail Research, Texas A &
M University-Kingsville, 700 University Boulevard, MSC 218, Kingsville,
TX 78363-8202, USA
Abstract
We describe polymerase chain reaction (PCR) primers and conditions to
amplify eight dinucleotide, one trinucleotide and 14 tetranucleotide microsatellite
DNA loci isolated from the northern bobwhite (Colinus virginianus).
The PCR primers were tested on 16 individuals collected from a population
located within the Red Hills region of south Georgia and north Florida.
The 23 primer pairs developed in this study yielded an average of 6.5
alleles per locus (range 2-11), an average observed heterozygosity of
0.47 (range 0.06-0.94) and average polymorphic information content of
0.60 (range 0.06-0.85).
Keywords: Colinus virginianus, Galliformes,
microsatellites, PCR, primer, quail, SSR
Received
26 January 2004; revision received 22 March 2004; accepted 22 March 2004
SREL Reprint #2782
Schable, N. A., B. C. Faircloth, W. E. Palmer, J. P. Carroll, L. W. Burger, L. A. Brennan, C. Hagen and T. C. Glenn. 2004. Tetranucleotide and dinucleotide microsatellite loci from the northern bobwhite (Colinus virginianus). Molecular Ecology 4:415-419.
