Characterization of six microsatellite primers for the grey fox (Urocyon cinereoargenteus)
JULIE L. WESTON,1,2 NANCY A. SCHABLE1 and TRAVIS
C. GLENN1,2
1Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC
29802, USA
2Peromyscus Genetic Stock Center, Department of Biological
Sciences, University of South Carolina, Columbia, SC 29208, USA
Abstract
We describe polymerase chain reaction (PCR) primers and conditions to
amplify one dinucleotide and five tetranucleotide microsatellite DNA loci
isolated from the grey fox (Urocyon cinereoargenteus). The PCR
primers were tested on nine to 12 individuals collected from the Department
of Energy's Savannah River site (Aiken, SC, USA). The grey fox microsatellite
primers developed had three to 10 alleles per locus that yielded observed
heterozygosities ranging from 0.222 to 0.889.
Keywords: dinucleotide repeats, grey fox, microsatellite, polymerase
chain reaction, primer, simple sequence repeat, tetranucleotide repeats,
Urocyon cinereoargenteus
Received 22 April 2004; revision accepted 18 May 2004
SREL Reprint #2838
Weston, J. L., N. A. Schable and T. C. Glenn. 2004. Characterization of six microsatellite primers for the grey fox (Urocyon cinereoargenteus). Molecular Ecology Notes 4:503-505.
