Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae)
ADRIANA OTERO-ARNAIZ,1 ANDREW SCHNABEL,1 TRAVIS
C. GLENN,2 NANCY A. SCHABLE,2 CRIS HAGEN2
and LEILA NDONG1
1Department of Biological Sciences, Indiana University South
Bend, PO Box 7111, South Bend, IN 46634, USA
2Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802,
USA
Abstract
We have developed primer pairs for 24 microsatellite loci isolated from
Acacia brevispica, which is widespread in woodland savannas of
East Africa. The loci were screened for levels of variation using 16-52
individuals from Mpala Research Centre, Kenya. Number of alleles per locus
ranged from two to 17, and polymorphic information content ranged from
0.248 to 0.864. Several loci showed the presence null alleles, but many
loci will be useful in ongoing studies of genetic structure, gene flow,
breeding systems, and natural selection.
Keywords: Acacia, gene flow, genetic structure, molecular markers,
PCR, SSR
Received
28 November 2004; revision accepted 14 January 2005
SREL Reprint #2840
Otero-Arnaiz, A., A. Schnabel, T. C. Glenn, N. A. Schable, C. Hagen and L. Ndong. 2005. Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae). Molecular Ecology Notes 5:366-368.
