Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus)
BRANT C. FAIRCLOTH,1 ALLISON REID,1 TERESA VALENTINE,1
SOO HYUNG EO,1 THERON M. TERHUNE,1 TRAVIS C. GLENN,2,3
WILLIAM E. PALMER,4 CAMPBELL JOSEPH NAIRN1 and JOHN
P. CARROLL1
1D. B. Warnell School of Forest Resources, University
of Georgia, Athens, GA, 30602, USA
2 Savannah River Ecology Laboratory, University of Georgia,
P.O. Drawer E, Aiken, SC 29802, USA
3Department of Biological Sciences, University of South Carolina,
Columbia, SC 29208, USA
4 Tall Timbers Research Station, 13093 Henry Beadel Drive,
Tallahassee, FL 32312, USA
Abstract
We describe primers and polymerase chain reaction (PCR) conditions to
amplify four dinucleotide, one trinucleotide, and three tetranucleotide
microsatellite DNA loci from the bobcat (Lynx rutus). The primers
were tested on 22 individuals collected from a population located within
southwestern Georgia (USA). The primer pairs developed in this study yielded
an average of 7.4 alleles per locus (range four to 10), an average observed
heterozy- gosity of 0.60 (range 0.40 to 0.76), and an average polymorphic
information content of 0.70 (range 0.51 to 0.78).
Keywords: bobcat, dinucleotide repeats, Lynx rufus,
microsatellites, primers, SSRs, tetranucleotide repeats, trinucleotide
repeats
Received 14 December 2004; revision accepted 26 January 2005
SREL Reprint #2841
Faircloth, B. C., A. Reid, T. Valentine, S. H. Eo, T. M. Terhune, T. C. Glenn, W. E. Palmer, C. J. Nairn and J. P. Carroll. 2005. Tetranucleotide, trinucleotide, and dinucleotide loci from the bobcat (Lynx rufus). Molecular Ecology Notes 5:387-389.
