Isolating Microsatellite DNA Loci
By TRAVIS C. GLENN and NANCY A. SCHABLE
Abstract
A series of techniques are presented to construct genomic DNA libraries
highly enriched for microsatellite DNA loci. The individual techniques
used here derive from several published protocols but have been optimized
and tested in our research laboratories as well as in classroom settings
at the University of South Carolina and University of Georgia, with students
achieving nearly 100% success. Reducing the number of manipulations involved
has been a key to success, decreasing both the failure rate and the time
necessary to isolate loci of interest. In our lab during the past 3 years
alone, these protocols have been successfully used to isolate microsatellite
DNA loci from at least 55 species representing three kingdoms. These protocols
have made it possible to reduce the time to identify candidate loci for
primer development from most eukaryotic species to as little as 1 week.
SREL Reprint #2870
Glenn, T. C. and N. A. Schable. 2005. Isolating microsatellite DNA loci. Methods in Enzymology 395:202-222.
