Cell
adhesion of Shewanella oneidensis to iron oxide minerals:
Effect of different single crystal faces
Andrew
L. Neal (a)
Savannah River Ecology Laboratory, University of Georgia, Aiken, South
Carolina 29808
Tracy L. Bank (b) and Michael F. Hochella, Jr.
Department of Geosciences, Virginia Polytechnic Institute and State University,
Blacksburg,
Virginia 24060
Kevin M. Rosso
The W.R. Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest
National Laboratory,
Richland, Washington 99352
Abstract
The results of experiments designed to test the hypothesis that near-surface
molecular structure of
iron oxide minerals influences adhesion of dissimilatory iron reducing
bacteria are presented. These
experiments involved the measurement, using atomic force microscopy, of
interaction forces
generated between Shewanella oneidensis MR-1 cells and single
crystal growth faces of iron oxide
minerals. Significantly different adhesive force was measured between
cells and the (001) face of
hematite, and the (100) and (111) faces of magnetite. A role for electrostatic
interactions is apparent.
The trend in relative forces of adhesion generated at the mineral surfaces
is in agreement with
predicted ferric site densities published previously. These results suggest
that near-surface structure
does indeed influence initial cell attachment to iron oxide surfaces;
whether this is mediated via
specific cell surface-mineral surface interactions or by more general
interfacial phenomena remains
untested. © 2005 American Institute of Physics. [DOI: 10.1063/1.2151110]
(a)Author
to whom correspondence should be addressed; electronic mail: neal@srel.edu
(b)Present address: Oak Ridge National Laboratory, Oak Ridge,
TN 37831.
SREL
Reprint #2911
Neal, A. L., T. L. Bank, M. F. Hochella, Jr. and K. M. Rosso 2005. Cell adhesion of Shewanella oneidensis to iron oxide minerals: effect of different single crystal faces. Geochemical Transactions 6:77-84.
