Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus
LYNNE M. MULLEN,* RACHEL J. HIRSCHMANN,* KELLY L. PRINCE,†‡
TRAVIS C. GLENN,‡§
MICHAEL J. DEWEY†‡ and HOPI E. HOEKSTRA*
*Division
of Biological Sciences, University of California San Diego, La Jolla,
CA 92093, USA,
†Peromyscus
Genetic Stock Center
‡Department of Biological Sciences, University of South Carolina,
Columbia, SC 29208, USA,
§Savannah
River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
Abstract
We isolated and characterized 60 novel microsatellite markers from the
closely related oldfield mouse (Peromyscus polionotus) and deer
mouse (Peromyscus maniculatus) for studies of conservation, ecological,
quantitative and population genetics. We assessed all 60 markers in a
wild population of Peromyscus polionotus rhoadsi (N = 20) from
central Florida and found an average of nine alleles per marker and an
observed heterozygosity (HO) of 0.66 (range = 0.00–1.00).
These polymorphic markers contribute to the growing number of genomic
resources for Peromyscus, an emerging model system for ecological
and evolutionary research.
Keywords: deer mouse, microsatellites, oldfield mouse, Peromyscus, Rodentia, simple sequence repeats
Correspondence: Hopi Hoekstra, Fax: 858-534-7108; E-mail: hoekstra@ucsd.edu
SREL Reprint #2932
Mullen, L. M., R. J. Hirschmann, K. L. Prince, T. C. Glenn, M. J. Dewey and H. E. Hoekstra. 2006. Sixty polymorphic microsatellite markers for the oldfield mouse developed in Peromyscus polionotus and Peromyscus maniculatus. Molecular Ecology Notes 6:36-40.
