Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin
Yong-Jin Lee1,3, Isaac D. Wagner1, Mary E. Brice1, Vadim V. Kevbrin1, Gary L. Mills3, Christopher S. Romanek2,3 and Juergen Wiegel1 *
(1)Department of Microbiology, University of Georgia, 527 Biological Sciences
Building, 1000 Cedar Street, Athens, GA 30602-2605, USA
(2) Department of Geology, University of Georgia, Athens, GA 30602, USA
(3) Savannah River Ecology Laboratory, Aiken, SC 29802, USA
Abstract
A new group of anaerobic thermophilic bacteria was isolated from enrichment
cultures obtained from deep sea sediments of Peru Margin collected during
Leg 201 of the Ocean Drilling Program. A total of ten isolates were obtained
from cores of 1-2 m below seafloor (mbsf) incubated at 60°C: three
isolates came from the sediment 426 m below sea level with a surface temperature
of 9°C (Site 1227), one from 252 m below sea level with a temperature
of 12°C (Site 1228), and six isolates under sulfate-reducing condition
from the lower slope of the Peru Trench (Site 1230). Strain JW/IW-1228P
from the Site 1228 and strain JW/YJL-1230-7/2 from the Site 1230 were
chosen as representatives of the two identified clades. Based on the 16S
rDNA sequence analysis, these isolates represent a novel group with Thermovenabulum
and Caldanaerobacter as their closest relatives. The temperature
range for growth was 52-76°C with an optimum at around 68°C for
JW/IW-1228P and 43-76°C with an optimum at around 64°C for JW/YJL-1230-7/2.
The pH25C range for growth was from 6.3 to 9.3 with an optimum
at 7.5 for JW/IW-1228P and from 5 to 9.5 with an optimum at 7.9-8.4 for
JW/YJL-1230-7/2. The salinity range for growth was from 0% to 6% (w/v)
for JW/IW-1228P and from 0% to 4.5% (w/v) for JW/YJL-1230-7/2. The G+C
content of the DNA was 50 mol% for both JW/IW-1228P and JW/YJL-1230-7/2.
DNA-DNA hybridization yielded 52% similarity between the two strains.
According to 16S rRNA gene sequence analysis, the isolates are located
within the family, Thermoanaerobacteriaceae. Based on their morphological
and physiological properties and phylogenetic analysis, it is proposed
that strain JW/IW-1228PT is placed into a novel taxa, Thermosediminibacter
oceani, gen. nov., sp. nov. (DSM 16646T=ATCC BAA-1034T),
and JW/YJL-1230-7/2T into Thermosediminibacter litoriperuensis
sp. nov. (DSM 16647T =ATCC BAA-1035T).
Keywords: Ocean Drilling Program (ODP), Leg 201, Thermosediminibacter oceani, Thermosediminibacter litoriperuensis, Deep sea sediment
*Juergen
Wiegel
Email: jwiegel@uga.edu
Phone: +1-706-5422651
Fax: +1-706-5422651
SREL Reprint #2994
Lee, Y. J., I. D. Wagner, M. E. Brice, V. V. Kevbrin, G. Mills, C. S. Romanek and J. Wiegel. 2005. Thermosediminibacter oceani gen. nov., sp. nov. and Thermosediminibacter litoriperuensis sp. nov., new anaerobic thermophilic bacteria isolated from Peru Margin. Extremophiles 9:375-383.
