Thirteen
polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis
(Teiidae: Squamata) and related cnemidophorine lizards
Nicholas G. Crawford1,3, Jaime Zaldívar-Rae2,
Cris Hagen3, Amanda Schable3,
Erica Bree Rosenblum4,5, Jeff A. Graves6, Tod
W. Reeder1, Michael G. Ritchie6, and
Travis C. Glenn3
1Department
of Biology, San Diego State University, San Diego, CA 92182–4614,
USA
2Laboratorio de Conducta Animal, Instituto de Ecología,
Universidad Nacional Autónoma de México,
A.P. 70–275, C.P. 04510, México, D.F. Mexico
3Savannah River Ecology Laboratory, University of Georgia,
Drawer E, Aiken, SC 29802, USA
4Department of Molecular and Cell Biology, University of
California at Berkeley, Berkeley, CA 94720, USA
5Department of Genome Sciences, Lawrence Berkeley National
Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA
6Environmental and Evolutionary Biology, University of St.
Andrews, KY16 9TS, UK
Abstract:
We describe polymerase chain reaction primers and amplification conditions
for 13 microsatellite DNA loci isolated from two bisexual species of whiptail
lizards Aspidoscelis costata huico and Aspidoscelis inornata.
Primers were tested on either 16 or 48 individuals of A. c. huico
and/or 26 individuals of A. inornata. Ten of the 13 primers were
also tested against a panel of 31 additional whiptail taxa. We detected
three to nine alleles per locus in A. c. huico and four to 19 alleles
per locus in A. inornata, with observed heterozygosity ranging
from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will
be an important resource for surveys of genetic variation in these lizards.
Keywords:
Ameiva, Aspidoscelis, Cnemidophorus, lizard, microsatellite,
PCR primers, SSR, STR, teiid,
whiptail
SREL Reprint #3072
Crawford,
N. G., J. Zaldívar-Rae, C. Hagen, A. Schable, E. B. Rosenblum,
J. A. Graves, T. W. Reeder, M. G. Ritchie and T. C. Glenn. 2008. Thirteen
polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis
(Teiidae: Squamata) and related cnemidophorine lizards. Molecular Ecology
Resources 2008(8): 219-223.
