Isolation
and characterization of tetranucleotide microsatellite markers in a
mouth-brooding haplochromine cichlid fish (Pseudocrenilabrus multicolor
victoriae) from Uganda
Erika Crispo1, Cris Hagen2, Travis Glenn2,
Geneviève Geneau3, and Lauren J. Chapman1
1Department
of Biology, McGill University, 1205 Ave. Docteur Penfield, Montréal,
Québec, Canada,
H3A 1B1
2Savannah River Ecology Laboratory, University of Georgia,
Savannah River Site, Aiken, South Carolina, 29802, USA
3Génome Québec Innovation Centre, McGill University,
740 Ave. Docteur Penfield, Montréal, Québec, Canada, H3A
1A4
Abstract:
Eight tetranucleotide microsatellite loci were isolated from the haplochromine
cichlid fish, Pseudocrenilabrus multicolor victoriae, an important
model species for studies in respiratory ecology, conservation, and evolution.
We surveyed variation at these loci in 23 individuals from western Uganda,
finding four to 19 alleles per locus and an average expected heterozygosity
of 0.8575. These microsatellite loci will be used to examine gene flow
and population structure in Ugandan P. m. victoriae.
Keywords:
African cichlid, enrichment, population genetics, primer design, sequencing,
tetranucleotide
SREL Reprint #3073
Crispo, E.,
C. Hagen, T. C. Glenn, G. Geneau and L. J. Chapman. 2007. Isolation and
characterization of tetranucleotide microsatellite markers in a mouth-brooding
haplochromine cichlid fish (Pseudocrenilabrus multicolor victoriae)
from Uganda. Molecular Ecology Notes 2007(7): 1293-1295.
