Development and characterization of microsatellite loci in the eastern chipmunk (Tamias striatus)

Development and characterization of microsatellite loci in the eastern chipmunk (Tamias striatus)

MAUREEN B. PETERS¹, JULIE L. GLENN¹, PAM SVETE¹, CRIS HAGEN¹, OLGA V. TSYUSKO¹,
PATRICIA DECOURSEY², MÉLISSA LIEUTENANT-GOSSELIN³, DANY GARANT³, and
TRAVIS C. GLENN^1,2

¹Savannah River Ecology Laboratory, Institute of Ecology, The University of Georgia, PO Drawer E, Aiken, SC 29802, USA
²Department of Biological Sciences, University of South Carolina, Columbia, SC 29802, USA
³Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1K 2R1

Abstract: We isolated and characterized 16 microsatellite loci in the eastern chipmunk, Tamias striatus. The loci were screened across 25 individuals from one population and shown to be polymorphic with the number of alleles per locus ranging from two to 17. Polymorphic information content ranged from 0.212 to 0.887 and observed heterozygosity ranged from 0.200 to 0.960. Only two loci (Chip 10 and Chip 25) deviated from Hardy–Weinberg equilibrium.

Keywords: Eastern chipmunk, microsatellites, parentage analysis, PCR, population structure, Tamias striatus

SREL Reprint #3074

Peters, M. B., J. L. Glenn, P. Svete, C. Hagen, O. V. Tsyusko, P. Decoursey, M. Lieutenant-Gosselin, D. Garant and T. C. Glenn. 2007. Development and characterization of microsatellite loci in the eastern chipmunk (Tamias striatus). Molecular Ecology Notes 2007(7): 877-879.