Fifteen
polymorphic microsatellite DNA loci from Hawaii’s Metrosideros
polymorpha (Myrtaceae: Myrtales), a model species for ecology and
evolution
NICHOLAS G. CRAWFORD1, CRIS HAGEN1, HEATHER F.
SAHLI2, ELIZABETH A. STACY2,
and TRAVIS C. GLENN1
1Savannah
River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC
29802, USA
2Department of Biology, University of Hawaii at Hilo, 200
W. Kawili Street, Hilo, HI 96720, USA
Abstract:
We developed 15 polymorphic microsatellite loci from the Hawaiian tree
Metrosideros polymorpha. These loci were screened against two varieties
from several populations and from 23 individuals from one mid-elevation
population on Hawaii Island. Loci were variable with the number of alleles
per locus ranging from three to 24. Polymorphic information content ranged
from 0.222 to 0.941, and observed heterozygosity ranged from 0.261 to
0.955.
Keywords:
Hawaii, Metrosideros, microsatellite, PCR primers, polymorpha,
SSR, STR, tree
SREL Reprint #3075
Crawford,
N. G., C. Hagen, H. F. Sahli, E. A. Stacy and T. C. Glenn. 2008. Fifteen
polymorphic microsatellite DNA loci from Hawaii's Metrosideros polymorpha
(Myrtaceae: Myrtales), a model species for ecology and evolution. Molecular
Ecology Resources 2008(8): 308-310.
