Isolation
and characterization of microsatellite loci for Florida largemouth bass,
Micropterus salmoides floridanus, and other micropterids
DIJAR J. LUTZ-CARRILLO1, CRIS HAGEN2, LUCY A.
DUECK2, and TRAVIS C. GLENN2
1A.
E. Wood Laboratory, Texas Parks & Wildlife Department, 507 Staples
Road, San Marcos, TX 78666, USA
2Savannah River Ecology Laboratory, University of Georgia,
PO Drawer E, Aiken, SC 29802, USA
Abstract:
We isolated and characterized 52 novel microsatellite markers from Florida
largemouth bass, Micropterus salmoides floridanus, for use in conservation,
management and population genetic studies. Markers were assessed in M.
s. floridanus from peninsular Florida (n = 30) and averaged
eight alleles per locus with observed heterozygosity of 0.57 (range 0–0.97).
Cross-taxa amplification was successful among 88% of tested congeners.
These polymorphic and potentially taxon-diagnostic markers contribute
to the limited number of microsatellites currently available for micropterids
and specifically M. s. floridanus.
Keywords:
Centrarchidae, Florida largemouth bass, micropterids, Micropterus,
microsatellites, simple sequence repeats
SREL Reprint #3077
Lutz-Carrillo,
D. J., C. Hagen, L. A. Dueck and T. C. Glenn. 2008. Isolation and characterization
of microsatellite loci for Florida largemouth bass, Micropterus salmoides
floridanus, and other micropterids. Molecular Ecology Resources 2008(8):
178-184.
