Microsatellite markers isolated from barn swallows (Hirundo rustica)

Microsatellite markers isolated from barn swallows
(Hirundo rustica)

OLGA V. TSYUSKO¹, MAUREEN B. PETERS¹, CRIS HAGEN¹, TRACEY D. TUBERVILLE¹, TIMOTHY
A. MOUSSEAU², ANDERS P. MØLLER³, and TRAVIS C. GLENN^1,2

¹The University of Georgia, Institute of Ecology, Savannah River Ecology Laboratory, PO Drawer E, Aiken, SC 29802, USA
²Department of Biological Sciences, University of South Carolina, Columbia, SC 29802, USA
³Laboratoire de Parasitologie Evolutive, Université Pierre et Marie Curie, FR-75252 Paris, Cedex 05, France

Abstract: Fifteen polymorphic microsatellite DNA loci were isolated from barn swallows (Hirundo rustica) and optimized for future studies of radiation-induced mutations in populations from Ukraine. The loci were screened for variability among 25 individuals from two populations. The primers amplified loci with relatively high numbers of alleles ranging from five to 32 alleles per locus and polymorphic information content from 0.481 to 0.951. Observed heterozygosity varied from 0.458 to 0.960. None of the loci showed deviations
from Hardy–Weinberg equilibrium in either population.

Keywords: Hirundo rustica, microsatellites, mutation, PCR, primer, radiation

SREL Reprint #3078

Tsyusko, O. V., M. B. Peters, C. Hagen, T. D. Tuberville, T. A. Mousseau, A. P. Moller and T. C. Glenn. 2007. Microsatellite markers isolated from barn swallows (Hirundo rustica). Molecular Ecology Notes 2007(7): 833-835.