Microsatellite
markers isolated from saltgrass (Distichlis spicata)
OLGA V. TSYUSKO1, MAUREEN B. PETERS1, TRACEY D.
TUBERVILLE1, CRIS HAGEN1,
SARAH M. EPPLEY2, and TRAVIS C. GLENN1,3
1Savannah
River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken,
SC 29802, USA
2Department of Biology, Portland State University, PO Box
751 Portland, OR 97207, USA
3Department of Biological Sciences, University of South Carolina,
Columbia, SC 29802, USA
Abstract:
Twelve polymorphic microsatellite DNA loci were isolated from saltgrass
(Distichlis spicata) and optimized for future studies of its breeding
system. The loci were screened for variability among 24 individuals from
two populations. The primers amplified loci with numbers of alleles ranging
from four to 14 per locus and polymorphic information content from 0.481
to 0.951. Observed heterozygosity varied from 0.227 to 0.958.
Keywords:
breeding system, Distichlis spicata, microsatellites, mutation,
PCR, primer
SREL Reprint #3079
Tsyusko,
O. V., M. B. Peters, T. D. Tuberville, C. Hagen, S. M. Eppley and T. C.
Glenn. 2007. Microsatellite markers isolated from saltgrass (Distichlis
spicata). Molecular Ecology Notes 2007(7): 883-885.
