Isolation of microsatellite loci from the coqui frog, Eleutherodactylus coqui

Isolation of microsatellite loci from the coqui frog, Eleutherodactylus coqui

MAUREEN B. PETERS¹, KAREN H. BEARD², CRIS HAGEN¹, ERIC M. O’NEILL³, KAREN E. MOCK²,
WILLIAM C. PITT⁴, and TRAVIS C. GLENN¹

¹Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA
²Department of Wildland Resources and the Ecology Center, Utah State University, Logan, UT 84322-5230, USA
³Department of Biology, Utah State University, Logan, UT 84322-5305, USA
⁴USDA/APHIS/WS/National Wildlife Research Center, Hilo Field Station, HI 96721, USA

Abstract: Thirteen microsatellite loci were isolated from the coqui frog (Eleutherodactylus coqui) and optimized for future research. The loci were screened across 37 individuals from two Puerto Rican populations. Loci were variable with the number of alleles per locus ranging from three to 38. Polymorphic information content ranged from 0.453 to 0.963 and observed heterozygosity for each population ranged from 0.320 to 0.920.

Keywords: amphibian, conservation genetics, invasive species, population structure, primer, Puerto Rico

SREL Reprint #3081

Peters, M. B., K. H. Beard, C. Hagen, E. M. O'Neill, K. E. Mock, W. C. Pitts and T. C. Glenn. 2008. Isolation of microsatellite loci from the coqui frog, Eleutherodactylus coqui. Molecular Ecology Resources 2008(8): 139-141.