Isolation
and characterization of microsatellite loci in the Guanacaste tree,
Enterolobium cyclocarpum
MAUREEN
B. PETERS1, CRIS HAGEN1, DORSET W. TRAPNELL2,
J. L. HAMRICK3,
OSCAR ROCHA4, PETER E. SMOUSE5, and TRAVIS C.
GLENN1
1Savannah
River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken,
SC 29802, USA
2Institute of Ecology, University of Georgia, Athens, GA
30602, USA
3Department of Plant Biology, University of Georgia, Athens,
GA 30602, USA
4Department of Biological Sciences, Kent State University,
Kent, OH 44242, US
5Ecology, Evolution and Natural Resources, Rutgers University,
New Brunswick, NJ 08901, USA
Abstract:
We isolated nine microsatellite loci from the Guanacaste tree (Enterolobium
cyclocarpum) and optimized them for future research on breeding populations
of this species. Loci were screened across 53 individuals from one population
and were shown to be variable with the number of alleles per locus ranging
from five to 15. Polymorphic information content ranged from 0.420 to
0.900 and observed heterozygosity ranged from 0.547 to 0.906.
Keywords:
Enterolobium cyclocarpum, Guanacaste tree, microsatellites,
parentage analysis, PCR, pollen dispersal, population structure
SREL Reprint #3082
Peters, M.
B., C. Hagen, D. W. Trapnell, J. L. Hamrick, O. Rocha, P. E. Smouse and
T. C. Glenn. 2008. Isolation and characterization of microsatellite loci
in the Guanacaste tree, Enterolobium cyclocarpum. Molecular Ecology
Resources 2008(8): 129-131.
