Isolation of polymorphic microsatellite markers in the sub-Saharan tree, Acacia (Senegalia) mellifera (Fabaceae: Mimosoideae)

Isolation of polymorphic microsatellite markers in the sub-Saharan tree, Acacia (Senegalia) mellifera (Fabaceae: Mimosoideae)

JUAN C. RUIZ-GUAJARDO¹, ADRIANA OTERO-ARNAIZ², TRAVIS TAYLOR³, GRAHAM STONE¹,
TRAVIS C. GLENN⁴, NANCY A. SCHABLE⁴, JOSEPH T. MILLER⁵, SONJA PREUSS¹, and
ANDREW SCHNABEL³

¹Institute of Evolutionary Biology, Kings Buildings, The University of Edinburgh, Edinburgh EH9 3JT, UK
²National Institute of Ecology, Periferico Sur 5000, Insurgentes Cuicuilco, Coyoacán, Mexico City 04530, Mexico
³Department of Biological Sciences, Indiana University South Bend, South Bend, IN 46634, USA
⁴Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA
⁵Roy J. Carver Center for Comparative Genomics, Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USA

Abstract: We isolated 11 polymorphic microsatellite markers from Acacia mellifera, a savannah woodland tree in sub-Saharan Africa and southern Arabia. The loci were screened for polymorphism using 48 Kenyan individuals. Allelic diversity ranged from three to 19 per locus and the polymorphic information content varied from 0.287 to 0.893. These loci will be useful in studies of genetic structure, gene flow and breeding systems.

Keywords: Acacia mellifera, Fabaceae, microsatellites, molecular markers, Senegalia

SREL Reprint #3083

Ruiz-Guajardo, J. C., A. Otero-Arnaiz, T. Taylor, G. Stone, T. C. Glenn, N. A. Schable, J. T. Miller, S. Preuss and A. Schnable. 2007. Isolation of polymorphic microsatellite markers in the sub-Saharan tree, Acacia (Senegalia) mellifera (Fabaceae: Mimosoideae). Molecular Ecology Notes 2007(7): 1138-1140.