Microsatellite
markers isolated from polyploid wood-sorrel, Oxalis alpina (Oxalidaceae)
OLGA V.
TSYUSKO1, TRACEY D. TUBERVILLE1, MAUREEN B. PETERS1,
NICHOLAS CRAWFORD1,
CRIS HAGEN1, STEPHEN G. WELLER2, ANN K. SAKAI2,
and TRAVIS C. GLENN1
1Savannah
River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken,
SC 29802, USA
2Department of Ecology and Evolutionary Biology, University
of California-Irvine, Irvine, CA 92697, USA
Abstract:
Twelve polymorphic microsatellite loci were isolated from polyploid alpine
wood-sorrel, Oxalis alpina (Oxalidaceae), and optimized for future
studies of its breeding system. The loci were screened for variability
among 72 individuals from Pinos Altos, New Mexico. The primers amplified
loci with allele number ranging from two to 17 per locus and with estimates
of Nei’s genetic diversity varying from 0.10 to 0.99. These primers
provide an opportunity to use polymorphic DNA markers to study the causes
of breeding system variability in this species.
Keywords:
breeding system, microsatellites, Oxalis alpina, PCR, polymorphism,
polyploidy, primer
SREL Reprint #3085
Tsyusko,
O. V., T. D. Tuberville, M. B. Peters, N. G. Crawford, C. Hagen, S. G.
Weller, A. K. Sakai and T. C. Glenn. 2007. Microsatellite markers isolated
from polyploid wood-sorrel, Oxalis alpina (Oxalidaceae). Molecular
Ecology Notes 2007(7): 1284-1286.
