253 Novel polymorphic microsatellites for the saltwater crocodile (Crocodylus porosus)
Lee G. Miles1, Sally R. Isberg1,2, Chris Moran1, Cris Hagen3, and Travis C. Glenn3,4
1Faculty
of Veterinary Science, University of Sydney, Room 513, RMC Gunn Building,
Sydney, NSW 2006, Australia
2Porosus Pty Ltd, P.O. Box 86, Palmerston, NT 0831, Australia
3Savannah River Ecology Laboratory, University of Georgia,
P.O. Drawer E, Aiken, SC 29802, USA
4Department of Environmental Health Science, University of
Georgia, Athens, GA 30602, USA
Abstract:
Genomic elucidation and mapping of novel organisms requires the generation
of large genetic resources. In this study, 253 novel and polymorphic microsatellite
loci were isolated and characterized for the saltwater crocodile (Crocodylus
porosus) by constructing libraries enriched for microsatellite DNA.
All markers were evaluated on animals obtained from Darwin Crocodile Farm
in the Northern Territory, Australia, and are intended for future use
in the construction of a genetic-linkage map for the saltwater crocodile.
The 253 loci yielded an average of 4.12 alleles per locus, and those selected
for mapping had an average polymorphic information content (PIC) of 0.425.
Keywords: Estuarine crocodile, Reptile, SSRs, Primers, Tetra-nucleotide
repeats, Tri-nucleotide repeats, Di-nucleotide repeats, Enrichment
SREL Reprint #3109
Miles, L.
G., S. R. Isberg, C. Moran, C. Hagen, and T. C. Glenn. 2009. 253 Novel
polymorphic microsatellites for the saltwater crocodile (Crocodylus
porosus). Conservation Genetics 10(2009): 963-980.
