Polymorphic microsatellite loci from Sprague’s pipit (Anthus spragueii), a grassland endemic passerine bird
NICHOLAS
G. CRAWFORD1, MAUREEN B. PETERS1, CRIS HAGEN1,
TRAVIS C. GLENN2,1,
STEPHEN K. DAVIS3,4, and CHRISTOPHER M. SOMERS4
1Savannah
River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC
29802, USA
2Department of Environmental Health Science, University of
Georgia, Athens, GA 30602, USA
3Canadian Wildlife Service, 300-2365 Albert Street, Regina,
Saskatchewan, Canada S4P 4K1
4Department of Biology, University of Regina, Regina, Saskatchewan,
Canada S4S 0A2
Abstract:
We
developed 11 novel microsatellite loci for Sprague’s pipit (Anthus
spragueii), a songbird endemic to the Northern Great Plains of central
North America. These loci were screened in 21 individuals from southern
Saskatchewan, Canada. All 11 loci were polymorphic, with the number of
alleles per locus ranging from five to 23, polymorphic information content
from 0.312 to 0.947, and observed heterozygosity from 0.476 to 0.900.
These loci will be suitable for a wide variety of studies on pipit natural
history, ecology, and conservation.
Key words: Anthus spragueii, microsatellite, PCR primers, Sprague’s
pipit, SSR, STR
SREL Reprint #3112
Crawford,
N. G., M. B. Peters, C. Hagen, T. C. Glenn, S. K. Davis, and C. M. Somers.
2009. Polymorphic microsatellite loci from Sprague's pipit (Anthus
spragueii), a grassland endemic passerine bird. Molecular Ecology
Resources 9(2009): 315-317.
