Epifluorescence and Confocal Filter Sets available at the Center
Click here for chart of one-photon and two-photon fluorochrome database
Note: this is a database in progress and is subject to corrections, additions and modification.
There are three fluorescence microscopes available. Epifluorescence cube information provided by manufacturers (some Nikon filters manufactured by Chroma
1. Inverted Nikon TE300 with a Princeton CCD camera and conventional film camera attached
2. Nikon Optiphot attached to BioRad confocal system
3. Leica DMRX-E attached to Leica confocal system
Filter sets that can be used in either Nikon microscope are indicated with an asterisk.
Filters for NikonExcitationDichroic/BarrierEmission
*UV (DAPI/Hoechst/AMCA)D 340-380400 DCLPD 435-485
*FITC/GFPD 465-495505 DCLPD 515-555
*FITC/GFP450-490510 LP520-560
*TRITC/rhodamine528-552565 DCLP578-632
*TRITC/rhodamine510-650580 LP590
Endow cube (GFP specific)HQ 450-490Q 496 LPHQ 500-550
Filters for Leica
DAPI/Hoecsht340-380NA 425 LP
FITC/GFP450-490NA 515 LP
FITC restricted460-500NA512-542
TRITC/rhodamine515-560NA 590 LP

Filters for Confocal Laser Excitation
Note: Leica excitation/dichroic/emission detection is user defined
BIORAD confocal cubeExcit.
FITC/GFP 488
TRITC/Rhodamine568
CY5/far red688
LEICA laser Excit.Dichroics
Argon:458
478
488
514		RSP 500
458/514
RT 30/70
substrate
GreNe:543488/543
HeNe:633488/543/633
Two-photon laser tunable: 780-900 nm