Tetranucleotide micro satellite DNA loci from the dollar sunfish (Lepomis marginatus)

NANCY A. SCHABLE^1,2, ROBERT U. FISCHER² andTRAVIS C. GLENN¹

¹Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC 29802, USA,
²Deparlnzent of Biological Sciences, Eastern nlinois University, Charleston, IL 61920, USA

Abstract
We describe polymerase chain reaction (PCR) primers and conditions to amplify one dinucle- otide and eight tetranucleotide microsatellite DNA loci isolated from .the dollar sunfish (Lepomis marginatus). The PCR primers were tested on 20 or more individuals from five populations. The dollar sunfish microsatellite primers developed yielded a high number of alleles (4 to 14 per locus), and high observed heterozygosities

Keywords: dinucleotide repeats, Lepomis marginatus, microsatellite, PCR, primer, SSR, tetranucle- otide repeats

SREL Reprint #2669

Schable, N. A., R. U. Fischer and T. C. Glenn. 2002. Tetranucleotide microsatellite DNA loci from the dollar sunfish (Lepomis marginatus). Molecular Ecology Notes 2:509-511.  

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