JACEK GAERTIG
Professor
Ph.D., 1989
Warsaw University, Poland
RESEARCH
The ability of eukaryotic cells to adopt variable shapes, to move in different directions, or to distribute cell components within the cytoplasm is controlled by a network of filaments: the cytoskeleton. Microtubules are the major type of cytoskeletal filaments, consisting of linear polymers of tubulin proteins. Microtubules form a cytoplasmic network which provides intracellular tracks for organellar transport performed by molecular motors (such a kinesins and dyneins). Furthermore, microtubules provide an internal structural support for many complex organelles such as the centrosomes, basal bodies, mitotic spindle, cilia and flagella.
Our model organism, the ciliate Tetrahymena thermophila, labelled by two monoclonal antibodies: on which recognized polyglycylated tubulin (green) and another which recognizes beta-tubulin (red).
We are studying an unicellular organism, the ciliate Tetrahymena thermophila, which assemble at least 17 distinct microtubular structures in a single cell. Our goal is to determine how specific types of microtubular organelles are assembled and maintained. We use cell biological, biochemical and molecular genetic approaches. In particular, we exploit an approach of genetic transformation of Tetrahymena, which allows us to determine the function of a cloned cytoskeletal protein gene by performing a "gene knockout" in vivo. Current research projects include: 1) identification of molecular domains of tubulin proteins involved in adaptation of microtubules to specific functions, with particular emphasis on the role of post-translational tubulin modifications like acetylation, polyglycylation and polyglutamylation; 2) identification of tubulin domains involved in binding of drugs commonly used in cancer chemotherapy like paclitaxel; 2) studies on proteins involved in assembly of microtubular organelles; currently we study the Tetrahymena homologs of kinesin II, a conserved motor protein, involved in assembly of cilia, 4) we explore the potential of Tetrahymena as a vehicle for expression of foreign proteins of biotechnological importance on the cell surface, including the surface antigens of parasitic protists.
| CONTACT INFORMATION |
| (706) 542-3409, jgaertig@cb.uga.edu |
| SEE ALSO |
| Gaertig Lab Page Biomedical & Health Sciences Institute NSF Press Release --Researchers Identify Proteins that Direct Intracellular Transport and Locomotion |
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| Drashti Dave | |
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| Swati Suryavanshi | |
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| Krishna Vasudevan | |
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| Dorota Wloga | |
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REPRESENTATIVE PUBLICATIONS
Verhey,K., and Gaertig J. The Tubulin Code. (2007). Cell Cycle, 6: 2152-2160.
Sharma, N., Bryant J., Wloga D., Donaldson R., Davis R.C., Jerka-Dziadosz, M., and Gaertig J. Katanin regulates dynamics of microtubules and biogenesis of motile cilia. (2007). J. Cell Biology, 178: 1065-1079.
Reed, N.A., Cai, D., Blasius, T.L., Jih, G. T., Meyhofer, E., Gaertig, J., and Verhey K.J. (2006). Microtubule acetylation promotes kinesin-1 binding and transport. Current Biology. 16: 2166-2172.
Eisen J.A., , Coyne, R.S., Wu M., Wu, D., Thiagarajan, M., Wortman, J.R., Badger, J.H., Ren, Q., Delcher, A.L., Salzberg, S.L., Silva, J.C., Haas, J.C., Majoros, W.H., Farzad,M., Carlton, J.M., Garg, J., Pearlman, R.E., Karrer, K.M., Sun, L.M., Smith, R.K., Elde, L.C., Turkewitz, A.P., Asai, D.J., Wilkes, D.E., Wang, Y., Cai, H., Collins, K., Wilamowska, K., Ruzzo, W.L., Weinberg, Z., , B., Stewart, A., Lee, S.L., Wloga, D., Frankel, J., Gaertig, J., Tsao, C-C., Gorovsky, M.A., Cherry, J.M., Stover, N.A., Krieger, C.J., Hamilton, E.P., Orias, E. (2006). Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote. PLoS Biology. 4, e286.
Wloga, D., Camba, A., Rogowski, K. , G. Manning, Jerka-Dziadosz, M., and Gaertig, J. (2006). Members of the Nima-related kinase family promote disassembly of cilia by multiple mechanisms. Mol. Biol. Cell. 17: 2799-2810.
Janke, C., Rogowski K., Wloga, D., Regnard,C., Kajava, AV., Strub, J-M., Temurak, N., van Dijk,J., Boucher, D., van Dorsselar, A., Suryavanshi, S., Gaertig, J., and Edde B. (2005). Tubulin polyglutamylase enzymes are members of the TTL domain protein family. Science. 308: 1758-1762
Brown, J.M., Fine. N.A., Pandiyan G., Thazhath, R. and Gaertig J. 2003. "Hypoxia regulates assembly of cilia in suppressors of Tetrahymena lacking an intraflagellar transport subunit gene." Mol. Biol. Cell. 14: 3192-3207.
Thazhath R., C. Liu, and J. Gaertig. 2002. "Polyglycylation domain of b-tubulin maintains axonemal architecture and controls progression of cytokinesis in Tetrahymena." Nature Cell Biol. 4: 256-259.
Xia, L., B. Hai, Y. Gao, D. Burnette, R. Thazhath, J. Duan, M.H. Br, N. Levilliers, M.A. Gorovsky, and J. Gaertig. 2000. "Polyglycylation of tubulin is essential and affects cell motility and division in Tetrahymena thermophila." J. Cell. Biol. 149: 1097-1106.
Brown, J., C. Marsala, R. Kosoy, J. Gaertig. 1999. "Kinesin-II is preferentially targeted to assembling cilia and is required for ciliogenesis and normal cytokinesis in Tetrahymena." Mol. Biol. Cell. 10, 3081-3096.
Gaertig, J., Y. Gao, T.G. Clark, H.W. Dickerson. 1999. "Surface display of a parasite antigen in the ciliate. Tetrahymena thermophila." Nature-Biotechnology 17: 462-465.
Gaertig, J., M.A. Cruz, J. Bowen, L. Gu, and M.A. Gorovsky. 1995. "Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila." J. Cell Biol. 129: 1301-1310.